Template:Kyoto/Notebook/Aug 28

From 2013.igem.org

(Difference between revisions)
(→Ligation)
(→Gel Extraction: （editor:kojima）)
Line 51: Line 51:
===Gel Extraction===
===Gel Extraction===
 +
<!-- こっから -->
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Lane||DNA
 +
|-
 +
|1||100bpladder||--
 +
|-
 +
|3||SasA(8/27 Genome PCR production)
 +
|-
 +
|5||RpaA(8/27 Genome PCR production)
 +
|-
 +
|7||RpaB(8/27 Genome PCR production)
 +
|-
 +
|9||PkaiBC(8/27 Genome PCR production)
 +
|}
 +
[[File:igku_xxbeforexx.xxx]]<br>
 +
[[File:igku_xxafterxx.xxx]]<br>
 +
===Colony PCR===
===Colony PCR===
===Restriction Enzyme Digestion===
===Restriction Enzyme Digestion===

Revision as of 06:08, 21 September 2013

Contents

Aug 28

Electrophoresis

No name

LaneSample
1100bp
2SasA
3RpaA
4RpaB
5PkaiBC
6PCR NC
7100bp

File:Igku xxxxxx.xxx

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
8/27 Plux173.81.951.45

Ligation

No name

stateVectorInserter
experiment8/71 DT-13.08.0
  • Samples were evaporeted used evaporator into about 3 µL.
sampleMilliQLigation Hightotal
343.510.5
  • incubate one hour at 16 °C

Gel Extraction

No name

LaneDNA
1100bpladder--
3SasA(8/27 Genome PCR production)
5RpaA(8/27 Genome PCR production)
7RpaB(8/27 Genome PCR production)
9PkaiBC(8/27 Genome PCR production)

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Colony PCR

Restriction Enzyme Digestion

Electrophoresis

Gel Extraction

Colony PCR

Liquid Culture

Ligation

===Liquid Culture===
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Aug_28"