Team:UNIK Copenhagen/Signe Notebook

From 2013.igem.org

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<h1>Week 1</h1>
<h1>Week 1</h1>
<p> 8<sup>th</sup>-14<sup>th</sup> of July </p>
<p> 8<sup>th</sup>-14<sup>th</sup> of July </p>
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<p>This week the team . . .</p>
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<p>
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<b>Culturing of magnetotactic bacteria (MTBs)</b> <br>
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Making base media for growing magnetotactic bacteria. This media was also used to make agar plates.
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MS-1 inoculated in liquid base medium. Falcon tubes were flushed with the nitrogen before use and incubated at 28 degrees.
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Plates were kept in a  closed container with an Oxoid AnaeroGen pad.<br><br>
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<b>Assembly of constructs</b> <br>
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Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates.
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Primers for MamC (MS-1 strain) was ordered.  
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<div class="right_page">

Revision as of 19:14, 21 September 2013

Notebook

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Week 1

8th-14th of July

Culturing of magnetotactic bacteria (MTBs)
Making base media for growing magnetotactic bacteria. This media was also used to make agar plates. MS-1 inoculated in liquid base medium. Falcon tubes were flushed with the nitrogen before use and incubated at 28 degrees. Plates were kept in a closed container with an Oxoid AnaeroGen pad.

Assembly of constructs
Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates. Primers for MamC (MS-1 strain) was ordered.

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Week 2

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Week 3

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Week 4

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Week 5

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Week 6

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Week 7

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Week 8

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Week 9

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Week 10

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Week 11

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Week 12

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