Template:Kyoto/Notebook/Aug 29

(Difference between revisions)

Revision as of 19:39, 22 September 2013

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Sample	base pair
8/27 Pcon-pT181 antisense-(1)	542
8/26 pT181 attenuator-DT-(1)	738

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	30s	30cycles

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Lane	Sample
1	100bp ladder
2	8/21 RBS-lysis2-9(Colony PCR prodution)
3	8/21 RBS-lysis2-8(Colony PCR prodution)
4	8/21 RBS-lysis2-7(Colony PCR prodution)
5	8/21 RBS-lysis2-10(Colony PCR prodution)
6	8/21 RBS-lysis2-11(Colony PCR prodution)
7	8/21 RBS-lysis2-12(Colony PCR prodution)
8	8/21 RBS-lysis2-13(Colony PCR prodution)
9	8/21 RBS-lysis2-14(Colony PCR prodution)

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DNA	concentration [µg/mL]	260/280	260/230
Plac	153.7	2.03	2.10
Pλ-luxⅠ-(1)	76.5	2.07	2.06
Pλ-luxⅠ-(2)	86.4	2.08	2.10
DT	166.2	1.65	2.12
J23100	190.1	1.68	2.34

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	8/22 psB1C3-(2)	EcoRI	SpeI	XbaI	PstI	BufferB	BufferD	BSA	MilliQ	total
2 cuts	8µL	0.5µL	0.5µL	0µL	0µL	3µL	0µL	0.3µL	17.7µL	30µL
NC	1µL	0µL	0µL	0µL	0µL	1µL	0µL	0.1µL	7.9µL	10µL
2 cuts	8µL	0µL	0µL	0.5µL	0.5µL	0µL	3µL	0.3µL	17.7µL	30µL
NC	1µL	0µL	0µL	0µL	0µL	0µL	1µL	0.1µL	7.9µL	10µL

	8/21 tRMA-spinach(1)	XbaI	SpeI	BufferB	BSA	MilliQ	total
2 cuts	4µL	0.5µL	0.5µL	3µL	0.3µL	21.7µL	30µL
NC	0.5µL	0µL	0µL	1µL	0.1µL	8.4µL	10µL

	8/21 tetR aptamer12-1R(1)	EcoRI	SpeI	BufferB	BSA	MilliQ	total
2 cuts	3µL	0.5µL	0.5µL	3µL	0.3µL	22.7µL	30µL
NC	0.4µL	0µL	0µL	1µL	0.1µL	8.5µL	10µL

	8/21 PT181 attenuator-(2)	EcoRI	SpeI	XbaI	PstI	BufferB	BufferD	BSA	MilliQ	total
2 cuts	4µL	0.5µL	0.5µL	0µL	0µL	3µL	0µL	0.3µL	21.7µL	30µL
NC	0.5µL	0µL	0µL	0µL	0µL	1µL	0µL	0.1µL	8.4µL	10µL
2 cuts	4µL	0µL	0µL	0.5µL	0.5µL	0µL	3µL	0.3µL	21.7µL	30µL
NC	0.5µL	0µL	0µL	0µL	0µL	0µL	1µL	0.1µL	8.4µL	10µL

	8/21 PT181 antisense-(2)	EcoRI	SpeI	XbaI	PstI	BufferB	BufferD	BSA	MilliQ	total
2 cuts	10µL	0.5µL	0.5µL	0µL	0µL	3µL	0µL	0.3µL	15.7µL	30µL
NC	1µL	0µL	0µL	0µL	0µL	1µL	0µL	0.1µL	7.9µL	10µL
2 cuts	10µL	0µL	0µL	0.5µL	0.5µL	0µL	3µL	0.3µL	15.7µL	30µL
NC	1µL	0µL	0µL	0µL	0µL	0µL	1µL	0.1µL	7.9µL	10µL

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Name	Sample	Competent Cells	Total	Plate
8/27 pT181 attenuator-DT	2µL	20µL	22µL	CP
8/27 RBS-lysis3-DT	2µL	20µL	22µL	CP
8/28 RBS-lysis3-DT	2µL	20µL	22µL	CP
8/28 Pcon-RBS-GFP-DT-Pcon-RBS-LuxR-DT	2µL	20µL	22µL	Amp
8/28 RBS-lysis1-DT	2µL	20µL	22µL	CP
8/28 Plux-RBS-GFP-DT	2µL	20µL	22µL	CP
8/28 Spinach-DT	2µL	20µL	22µL	CP

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