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Template:Kyoto/Notebook/Sep 1
From 2013.igem.org
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==Sep 1== | ==Sep 1== | ||
| + | ===Transformation=== | ||
===Transformation=== | ===Transformation=== | ||
<div class="experiment"> | <div class="experiment"> | ||
| + | <span class="author">No name</span> | ||
| + | {| class="wikitable" | ||
| + | !Name||Sample||Competent Cells||Total||Plate | ||
| + | |- | ||
| + | |8/31 tRNA-Spinach(pSB1C3)||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 pT181 antisense(pSB1C3)||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 pT181 attenuator(pSB1C3)||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 pT181 antisense(pSB1C3)||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 pT181 attenuator(2)-DT||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 pT181 antisense-DT||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 tRNA-Spniach-DT||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 RBS-lysis2-DT||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 Pconst-RBS-tetR-DT||2µL||20µL||22µL||Amp | ||
| + | |- | ||
| + | |8/31 Plac-RBS-lacZα-DT||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 Ptet-RBS-lacZα-DT||2µL||20µL||22µL||CP | ||
| + | |- | ||
| + | |8/31 ||2µL||20µL||22µL||CP | ||
| + | |} | ||
</div> | </div> | ||
| + | |||
===PCR=== | ===PCR=== | ||
<div class="experiment"> | <div class="experiment"> | ||
Revision as of 00:08, 23 September 2013
Contents |
Sep 1
Transformation
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| 8/31 tRNA-Spinach(pSB1C3) | 2µL | 20µL | 22µL | CP |
| 8/31 pT181 antisense(pSB1C3) | 2µL | 20µL | 22µL | CP |
| 8/31 pT181 attenuator(pSB1C3) | 2µL | 20µL | 22µL | CP |
| 8/31 pT181 antisense(pSB1C3) | 2µL | 20µL | 22µL | CP |
| 8/31 pT181 attenuator(2)-DT | 2µL | 20µL | 22µL | CP |
| 8/31 pT181 antisense-DT | 2µL | 20µL | 22µL | CP |
| 8/31 tRNA-Spniach-DT | 2µL | 20µL | 22µL | CP |
| 8/31 RBS-lysis2-DT | 2µL | 20µL | 22µL | CP |
| 8/31 Pconst-RBS-tetR-DT | 2µL | 20µL | 22µL | Amp |
| 8/31 Plac-RBS-lacZα-DT | 2µL | 20µL | 22µL | CP |
| 8/31 Ptet-RBS-lacZα-DT | 2µL | 20µL | 22µL | CP |
| 8/31 | 2µL | 20µL | 22µL | CP |
