Team:Washington/iGEMVectors

From 2013.igem.org

(Difference between revisions)
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<p>
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<li><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb">pSB1C3(std).gb</li>
 
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<li><a href = "https://2013.igem.org/Team:Washington/HEAT_SHOCK_CHEM_TRANS">6) Heat shock/chemical competent transformation</a></li>
 
<table>
<table>
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   <th>Antibiotic</th>
   <th>Antibiotic</th>
   <th>Length</th>
   <th>Length</th>
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   <th>Map</th>
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   <th>Maps: Genbank / ApE files (rename .ape)</th>
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   <td>Chloramphenicol</td>
   <td>Chloramphenicol</td>
   <td>2070 bp</td>
   <td>2070 bp</td>
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   <td>Map: </td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb">pSB1C3(std).gb</td>
</tr>
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   <td>Kanamycin</td>
   <td>Kanamycin</td>
   <td>2750 bp</td>
   <td>2750 bp</td>
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   <td>Map</td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb">pSB1C3(std).gb</td>
</tr>
</tr>
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   <td>Ampicillin</td>
   <td>Ampicillin</td>
   <td>2037 bp</td>
   <td>2037 bp</td>
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   <td>Map</td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb">pSB1C3(std).gb</td>
</tr>
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   <td>Tetracycline</td>
   <td>Tetracycline</td>
   <td>3252 bp</td>
   <td>3252 bp</td>
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   <td>Map</td>
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   <td><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb">pSB1C3(std).gb</td>
</tr>
</tr>
</table>
</table>

Revision as of 04:19, 25 September 2013



iGEM Vector Information

Vector Copy Number Equivalent Antibiotic Length Maps: Genbank / ApE files (rename .ape)
pSB1C3 100-300 ColE1,pMB1(pUC19) Chloramphenicol 2070 bp pSB1C3(std).gb
pSB3K3 20-30 p15A(pMR101) Kanamycin 2750 bp pSB1C3(std).gb
pSB4A5 ~5 RepA(pSC101) Ampicillin 2037 bp pSB1C3(std).gb
pSB3T5 20-30 p15A(pMR101) Tetracycline 3252 bp pSB1C3(std).gb


Vector Equivalency Chart
Source Vector Origin Copy Number Antibiotic Biobrick Equivalent Antibiotic
pJT119b ColE1,pMB1 100-300 Chloramphenicol pSB1C3 Chloramphenicol
pPLPCB p15A 20-30 Spectinomycin pSB3T5 Tetracycline
pEO100c pSC101 ~5 Ampicillin pSB4A5 Ampicillin
pJT106b pSC101 ~5 Ampicillin pSB4A5 Ampicillin