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Team:Bielefeld-Germany/Labjournal/June
From 2013.igem.org
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| - | + | **4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67 | |
| - | + | **Dilution to PCR template: 4-2006-453: 5.5ng/µl | |
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Revision as of 07:59, 25 September 2013
June
Milesstones
1.Week
Organization
- iGEM-Team Bielefeld will support the ‘CeBiTec Pupil Academy’ from 26.-30. August with an own experiment.
MFC
Mediators
Cytochromes
Biosafety
Porines
2.Week
Organization
- Thanks to NEB Biolabs for the free iGEM kit with many useful laboratory things for all German iGEM teams.
- We are working on our first press release.
- Having a short radio contribution in the Bielefeld university campus radio (radio 87.9 hertz).
MFC
Mediators
Cytochromes
Biosafety
Porines
3.Week
<a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/#">Organization</a>
- Nothing organizational, empty, empty, empty space.
MFC
Mediators
Cytochromes
- Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C
- Isolation of genomic DNA from S. oneidensis
- 4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78
- 4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67
- Dilution to PCR template: 4-2006-453: 5.5ng/µl
Amplification of the mtrCAB cluster
Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
Clear Bands at the expected 5.2kb
PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
Lane2: 4-2106-451: 7.4 ng/µl
Lane5: 4-2106-451: 8.5 ng/µl
4.Week
Organization
- Let’s go to Lyon, flights are booked for the European jamboree in Lyon from 11.-13. October 2013.
- We will participate at ‘BioNRW pHD Student Convention’ in Düsseldorf at 13. July.
