"
Team:Bielefeld-Germany/Labjournal/June
From 2013.igem.org
(Difference between revisions)
| Line 99: | Line 99: | ||
<br><br><br><br> | <br><br><br><br> | ||
<br><br><br><br> | <br><br><br><br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
<div id="3.Week"> | <div id="3.Week"> | ||
| Line 104: | Line 109: | ||
</div> | </div> | ||
| - | + | ====Organization==== | |
| - | + | *Nothing organizational, empty, empty, empty space. | |
| - | + | ||
| - | + | ||
====MFC==== | ====MFC==== | ||
Revision as of 08:00, 25 September 2013
June
Milesstones
1.Week
Organization
- iGEM-Team Bielefeld will support the ‘CeBiTec Pupil Academy’ from 26.-30. August with an own experiment.
MFC
Mediators
Cytochromes
Biosafety
Porines
2.Week
Organization
- Thanks to NEB Biolabs for the free iGEM kit with many useful laboratory things for all German iGEM teams.
- We are working on our first press release.
- Having a short radio contribution in the Bielefeld university campus radio (radio 87.9 hertz).
MFC
Mediators
Cytochromes
Biosafety
Porines
3.Week
Organization
- Nothing organizational, empty, empty, empty space.
MFC
Mediators
Cytochromes
- Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C
- Isolation of genomic DNA from S. oneidensis
- 4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78
- 4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67
- Dilution to PCR template: 4-2006-453: 5.5ng/µl
Amplification of the mtrCAB cluster
Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
Clear Bands at the expected 5.2kb
PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
Lane2: 4-2106-451: 7.4 ng/µl
Lane5: 4-2106-451: 8.5 ng/µl
4.Week
Organization
- Let’s go to Lyon, flights are booked for the European jamboree in Lyon from 11.-13. October 2013.
- We will participate at ‘BioNRW pHD Student Convention’ in Düsseldorf at 13. July.
