Team:Washington/iGEMVectors
From 2013.igem.org
(Difference between revisions)
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- | Vector files are in ApE format saved as .gb files. The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments. | + | Vector files are in Genbank compatible ApE format saved as .gb files. <br> |
+ | The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments. | ||
</p> | </p> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>pJT119b</td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/8/82/UW_pJT119b.gb">pJT119b</td> |
<td>ColE1,pMB1</td> | <td>ColE1,pMB1</td> | ||
<td>100-300</td> | <td>100-300</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>pPLPCB</td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/4/4c/UW_pPLPCB%28S%29.gb">pPLPCB</td> |
<td>p15A</td> | <td>p15A</td> | ||
<td>20-30</td> | <td>20-30</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>pEO100c</td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/2/20/UW_pEO100c.gb">pEO100c</td> |
<td>pSC101</td> | <td>pSC101</td> | ||
<td>~5</td> | <td>~5</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>pJT106b</td> | + | <td><a href = "https://static.igem.org/mediawiki/2013/9/9e/UW_pJT106b.gb">pJT106b</td> |
<td>pSC101</td> | <td>pSC101</td> | ||
<td>~5</td> | <td>~5</td> | ||
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<td>Ampicillin</td> | <td>Ampicillin</td> | ||
</tr> | </tr> | ||
+ | <a href = ""> | ||
</table> | </table> | ||
Revision as of 18:49, 25 September 2013
Vector | Copy Number | Equivalent | Antibiotic | Length | Genbank / ApE files |
---|---|---|---|---|---|
pSB1C3 | 100-300 | ColE1,pMB1 (pUC19) | Chloramphenicol | 2070 bp | pSB1C3(std).gb |
pSB1A3 | 100-300 | ColE1,pMB1 (pUC19) | Ampicillin | 2155 bp | pSB1A3(std).gb |
pSB3K3 | 20-30 | p15A (pMR101) | Kanamycin | 2750 bp | pSB3K3(std).gb |
pSB4A5 | ~5 | RepA (pSC101) | Ampicillin | 2037 bp | pSB4A5(std).gb |
pSB3T5 | 20-30 | p15A (pMR101) | Tetracycline | 3252 bp | pSB3T5(std).gb |
The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.
Source Vector | Origin | Copy Number | Antibiotic | Biobrick Equivalent | Antibiotic |
---|---|---|---|---|---|
pJT119b | ColE1,pMB1 | 100-300 | Chloramphenicol | pSB1C3 | Chloramphenicol |
pPLPCB | p15A | 20-30 | Spectinomycin | pSB3T5 | Tetracycline |
pEO100c | pSC101 | ~5 | Ampicillin | pSB4A5 | Ampicillin |
pJT106b | pSC101 | ~5 | Ampicillin | pSB4A5 | Ampicillin |