Team:Washington/iGEMVectors

From 2013.igem.org

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   <th>Vector</th>
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Revision as of 02:03, 26 September 2013



iGEM Vector Information

Vector Copy Number Equivalent Antibiotic Length Genbank / ApE files
pSB1C3 100-300 ColE1,pMB1 (pUC19) Chloramphenicol 2070 bp pSB1C3(std).gb
pSB1A3 100-300 ColE1,pMB1 (pUC19) Ampicillin 2155 bp pSB1A3(std).gb
pSB3K3 20-30 p15A (pMR101) Kanamycin 2750 bp pSB3K3(std).gb
pSB4A5 ~5 RepA (pSC101) Ampicillin 2037 bp pSB4A5(std).gb
pSB3T5 20-30 p15A (pMR101) Tetracycline 3252 bp pSB3T5(std).gb
Vector files are in Genbank compatible ApE format saved as .gb files.
The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.


Vector Equivalency Chart
Source Vector Origin Copy Number Antibiotic Biobrick Equivalent Antibiotic Biobrick Name
pJT119b ColE1,pMB1 100-300 Chloramphenicol pSB1C3 Chloramphenicol pBB_GonG
pPLPCB p15A 20-30 Spectinomycin pSB3T5 Tetracycline pBB_PCB
pEO100c pSC101 ~5 Ampicillin pSB4A5 Ampicillin pBB_RoffG
pJT106b pSC101 ~5 Ampicillin pSB4A5 Ampicillin pBB_RonL