Team:Washington/60mmPetriDishAssay
From 2013.igem.org
(Difference between revisions)
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4. Take another 60 mm petri dish (spacer plate) and place it below the uncovered petri dish.</br> | 4. Take another 60 mm petri dish (spacer plate) and place it below the uncovered petri dish.</br> | ||
5. Place the stacked plates such that the bottom of the spacer plate aligns with the lighted circle in the app.</br> | 5. Place the stacked plates such that the bottom of the spacer plate aligns with the lighted circle in the app.</br> | ||
- | 6. Secure the plates with tape. Incubate the tablet and dishes on an orbital shaker (RPM?) for 16 hours at | + | 6. Secure the plates with tape. Incubate the tablet and dishes on an orbital shaker (RPM?) for 16 hours at 37C</br> |
7. Transfer 200 ul culture to a 96-well black, clear-bottom plate for analysis. </br> | 7. Transfer 200 ul culture to a 96-well black, clear-bottom plate for analysis. </br> | ||
8. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br> | 8. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br> |
Revision as of 02:47, 27 September 2013