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Team:NTU-Taida/Notebook/Journal/May
From 2013.igem.org
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{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar-calendar|Title=Journal|Month=May}}{{:Team:NTU-Taida/Templates/ContentStart}} | {{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar-calendar|Title=Journal|Month=May}}{{:Team:NTU-Taida/Templates/ContentStart}} | ||
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===Transform=== | ===Transform=== | ||
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RBS (B0030) | RBS (B0030) | ||
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===Inoculation & Incubation：LB broth(Amp)=== | ===Inoculation & Incubation：LB broth(Amp)=== | ||
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RBS (B0030) | RBS (B0030) | ||
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===Get Plasmid: Use Plasmid miniprep purification kit=== | ===Get Plasmid: Use Plasmid miniprep purification kit=== | ||
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RBS (B0030) | RBS (B0030) | ||
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===Digestion:=== | ===Digestion:=== | ||
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B0030v-RhlR | B0030v-RhlR | ||
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B0030-RhlR | B0030-RhlR | ||
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[[File:NTU-Taida-journal-May-5.jpg]] | [[File:NTU-Taida-journal-May-5.jpg]] | ||
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B0030-RhlR | B0030-RhlR | ||
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===Check:=== | ===Check:=== | ||
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[[File:NTU-Taida-journal-May-7.jpg]] | [[File:NTU-Taida-journal-May-7.jpg]] | ||
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===Get Plasmid:=== | ===Get Plasmid:=== | ||
Latest revision as of 21:46, 27 September 2013
Contents |
5/17
Transform
LB plate (Ampicillin resistance)
RhlR (Bba_C0171),
pRhlR (Bba_R0071),
LasR (Bba_C0179),
pLas (Bba_R0079),
mCherry (Bba_J06504),
mRFP (Bba_E1010),
GFPmut3 (Bba_E0040),
Pc (Bba_J23119),
double terminator (B0015),
RBS (B0030)
5/18
Inoculation & Incubation：LB broth(Amp)
RhlR (Bba_C0171),
pRhlR (Bba_R0071),
LasR (Bba_C0179),
pLas (Bba_R0079),
mCherry (Bba_J06504),
mRFP (Bba_E1010),
GFPmut3 (Bba_E0040),
Pc (Bba_J23119),
double terminator (B0015),
RBS (B0030)
5/19
Get Plasmid: Use Plasmid miniprep purification kit
RhlR (Bba_C0171),
pRhlR (Bba_R0071),
LasR (Bba_C0179),
pLas (Bba_R0079),
mCherry (Bba_J06504),
mRFP (Bba_E1010),
GFPmut3 (Bba_E0040),
Pc (Bba_J23119),
double terminator (B0015),
RBS (B0030)
5/22
Digestion:
B0030
mCherry
mGFP
RFP
LasR
RhlR
After digestion we operate gel electrophoresis:
Ligation overnight
B0030v-mCherry
B0030v-mGFP
B0030v-RFP
B0030 v-LasR
5/23
Transform:
B0030-mCherry
B0030-mGFP
B0030-RFP
B0030-LasR
5/27
Inoculation & Incubation：
LB broth(Amp), 5 tubes for each kind
B0030-mCherry
B0030-mGFP
B0030-RFP
B0030-LasR
5/28
Check:
B0030-mCherry
B0030-mGFP
B0030-RFP
B0030-LasR
B0030-RhlR
5/30
Get Plasmid:
Use Plasmid miniprep purification kit
B0030-mCherry
B0030-mGFP
B0030-RFP
B0030-LasR
B0030-RhlR
