Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/5.5 Bacteria Cultures, 25 mL

(Difference between revisions)

Latest revision as of 00:24, 28 September 2013

Phage Purification May - June Notebook: Experiments

Phage Purification

March-April

5.5 Bacteria Cultures, 25 mL

I) Purpose

Grow bacteria for later use in purification of phage.

II) Expected Outcome

Viable liquid cultures of E. coli, both W3110 and BL21

III) Reagants Used

W3110 and BL21 freezer stock

LB

IV) Actual Procedure

Infect 25 mL of LB with 1 loop-full of bacterial freezer stock and leave in shaker at 37^◦ overnight.

V) Results

Growth of BL21 liquid cultures of bacteria to infect and amplify amounts of phage. W3110 failed to grow, so the experiment will have to be repeated.

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-: [[Team:BYU_Provo/Phage_Purification|Overview]]
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 '''V) Results'''
-: Growth of liquid culture of BL 21. W3110 failed to grow. We will follow the exact steps to regrow a new liquid culture of W3110.
+: Growth of BL21  liquid cultures of bacteria to infect and amplify amounts of phage. W3110 failed to grow, so the experiment will have to be repeated.
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