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Team:DTU-Denmark/Methods
From 2013.igem.org
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[[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]] | [[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]] | ||
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| + | ==USER Cloning== | ||
| + | [[Team:DTU-Denmark/Methods/USER_Cloning_and_Transformation|USER Cloning and Transformation]] | ||
==Plasmid isolation== | ==Plasmid isolation== | ||
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==Determining concentration of nitrogen compounds== | ==Determining concentration of nitrogen compounds== | ||
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| + | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_1a|Experiment 1a]] -- Toxicity of Nitrite, Nitrate and Ammonium to ''E. coli'' | ||
| - | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/ | + | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_2|Experiment 2]] -- Anaerobic production of nitrous oxide from nitrite |
| - | + | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_4|Experiment 4]] -- Aerobic consumption of ammonium and hydroxylamine | |
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| - | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_4|Experiment 4]] | + | |
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==Other== | ==Other== | ||
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| + | [[Team:DTU-Denmark/Methods/Visualizing_GFP_in_the_periplasm|Visualizing GFP in the periplasm]] | ||
[[Team:DTU-Denmark/Methods/chemically_compentent_cells|Preparation of chemically competent cells]] | [[Team:DTU-Denmark/Methods/chemically_compentent_cells|Preparation of chemically competent cells]] | ||
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[[Team:DTU-Denmark/Methods/Autoclaving|Autoclaving]] | [[Team:DTU-Denmark/Methods/Autoclaving|Autoclaving]] | ||
| - | [[Team:DTU-Denmark/Methods/Calibrating Electrodes|Calibration]] | + | [[Team:DTU-Denmark/Methods/Making anoxic conditions|Sparging with N<sub>2</sub>]] |
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| + | [[Team:DTU-Denmark/Methods/Modified DM minimal medium|Modified DM minimal medium]] | ||
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| + | [[Team:DTU-Denmark/Methods/Calibrating Electrodes|Calibration of Electrodes]] | ||
[[Team:DTU-Denmark/Methods/Ammonium colorimetric measurements|Ammonium colorimetric measurements]] | [[Team:DTU-Denmark/Methods/Ammonium colorimetric measurements|Ammonium colorimetric measurements]] | ||
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[[Team:DTU-Denmark/Methods/Nitrite colorimetric measurements|Nitrite colorimetric measurements]] | [[Team:DTU-Denmark/Methods/Nitrite colorimetric measurements|Nitrite colorimetric measurements]] | ||
| - | + | [[Team:DTU-Denmark/Methods/rehydrating_primers|Rehydrating Primers]] | |
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| + | [[Team:DTU-Denmark/Methods/dpni_treatment|DpnI treatment]] | ||
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| + | [[Team:DTU-Denmark/Methods/Bioreactor|Bioreactor]] | ||
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| + | <div style="clear: both;"></div> | ||
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| + | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 12:31, 1 October 2013
Methods
Contents |
PCR
USER Cloning
USER Cloning and Transformation
Plasmid isolation
Plasmid isolation by ethanol precipitation
Determining concentration of nitrogen compounds
Experiment 1a -- Toxicity of Nitrite, Nitrate and Ammonium to E. coli
Experiment 2 -- Anaerobic production of nitrous oxide from nitrite
Experiment 4 -- Aerobic consumption of ammonium and hydroxylamine
Other
Visualizing GFP in the periplasm
Preparation of chemically competent cells
Ammonium colorimetric measurements
Nitrate colorimetric measurements
Nitrite colorimetric measurements
