Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.8 Mutagen Concentration Test - Forth Protocol
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: - 30uL of 5.3 phage stock was added to each centrifuge tube (add nothing to C). ''From 5.15 Titer Test on 5.3 T7 new Phage Stock, we know that 5.3 phage stock has 7E8 particles/20uL. Thus, to each of the test tubes, we are adding approximately 1.2E9 particles.'' | : - 30uL of 5.3 phage stock was added to each centrifuge tube (add nothing to C). ''From 5.15 Titer Test on 5.3 T7 new Phage Stock, we know that 5.3 phage stock has 7E8 particles/20uL. Thus, to each of the test tubes, we are adding approximately 1.2E9 particles.'' | ||
- | : - Incubate phage on shaker at 37C | + | : - Incubate phage on shaker at 37C for 3 hours. |
- | : - Liquid culture from the four test (excluding C) tubes is then centrifuged at 4000 rpm for 10 minutes to pellet cell debris. Supernatant containing phage particles is removed and | + | : - Added 1mL of chloroform to each test tube. Liquid culture from the four test (excluding C) tubes is then centrifuged at 4000 rpm for 10 minutes to pellet cell debris. Supernatant containing phage particles is removed and placed in a new tube. |
: - The stock solutions are now stored in 4 Celsius. | : - The stock solutions are now stored in 4 Celsius. |
Revision as of 16:15, 9 July 2013
Small Phage July - August Notebook: Experiments
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7.8 Mutagen Concentration Test - Fourth Protocol
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Applying the mutagen (7.8)
2) Titer to Determine Phage Concentration 1 (7.10)
V) Results
VI) Conclusion
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