Team:UNITN-Trento/Notebook/Labposts/06/65

From 2013.igem.org

(Difference between revisions)

Latest revision as of 07:59, 3 October 2013

{ "date" : "2013-06-28", "author" : "emil", "title" : "Purification of K823024 and K823026 and inocula of the GFPs E0840 and E0240", "content" : "In orde to caracterize the inducible promoters of the two B. subtilis backbone I have to amplify two reporters and ligate them into the backbones. So I did the miniprep of the 4 inocula(B.subtilis backbones) following the vacuum protocol then I have quantified the products with the following results:

Quantification

Sample	Quantification
BBa_K823024	334.4ng/µl
BBa_K823024	260.6ng/µl
BBa_K823026	306.7ng/µl
BBa_K823026	359.7ng/µl

Moreover I did the inocula of the 2 GFPs(BBa_E0240 and BBa_E0840)(4 inocula for each sample) from the plates previously done.", "tags" : "K823024-K823026" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-06-26",
+	"date" : "2013-06-28",
-	"author" : "fabio-bruno",
+	"author" : "emil",
-	"title" : "bacillus subtilis promoters and  plasmids (part 9)!!",
+	"title" : "Purification of K823024 and K823026 and inocula of the GFPs E0840 and E0240",
-	"content" : "<html>  in order to verify weather the plasmids have been inverted during miniprep purification protocol, or screening protocol or even before, during plating, we made the minipreps out of the remaining inocula and then screened them: the risults were the same, so probably we made a mistake during plating! Now everything is more clear!!In the afternoon we made the inoculum of the last plasmid ( K823025, sent right from the Part’s Registry Headquarters).</html>",
+	"content" : "<html>In orde to caracterize the inducible promoters of the two B. subtilis backbone I have to amplify two reporters and ligate them into the backbones. So I did the miniprep of  the 4 inocula(B.subtilis backbones) following the <a href=\"http://ita.promega.com/~/media/Files/Resources/ProtCards/Wizard%20Plus%20SV%20Minipreps%20DNA%20Purification%20System%20Quick%20Protocol.pdf\">vacuum protocol</a> then I have quantified the products with the following results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification|<html><table><tr><th>Sample</th><th>Quantification</th></tr><tr><td>BBa_K823024</td><td> 334.4ng/&micro;l</td></tr><tr><td>BBa_K823024</td><td> 260.6ng/&micro;l</td></tr><tr><td>BBa_K823026</td><td> 306.7ng/&micro;l</td></tr><tr><td>BBa_K823026</td><td> 359.7ng/&micro;l</td></tr></table></html>}}<html>Moreover I did the inocula of the 2 GFPs(BBa_E0240 and BBa_E0840)(4 inocula for each sample) from the plates previously done.</html>",
-	"tags" : " plasmidPxil-plasmidPspac - plasmidluxABCDE "
+	"tags" : "K823024-K823026"
 }