Team:UNITN-Trento/Notebook/Labposts/07/41
From 2013.igem.org
(Difference between revisions)
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{ | { | ||
- | "date" : "2013-07- | + | "date" : "2013-07-21", |
- | "author" : " | + | "author" : "thomas", |
- | "title" : " | + | "title" : "Ligation of AraC-pBAD + EFE and Venus", |
- | "content" : " <html> | + | "content" : "<html>This is the part three of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. The digestion products were ligated together following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Ligation\">this protocol</a>. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.</html>", |
- | "tags" : " | + | "tags" : "EFE-Venus" |
} | } |
Revision as of 08:36, 3 October 2013
{ "date" : "2013-07-21", "author" : "thomas", "title" : "Ligation of AraC-pBAD + EFE and Venus", "content" : "This is the part three of this experiment. The digestion products were ligated together following this protocol. The vector (AraC-pBAD + EFE) was ligated with 1:0 (control), 1:1 and 1:4 of insert (Venus). The ligation product was then transformed into NEB10b competent cells and plated on Cloramphenicol containing Petri dishes.", "tags" : "EFE-Venus" }