Team:UNITN-Trento/Notebook/Labposts/07/55

From 2013.igem.org

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{
{
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"date" : "2013-07-11",
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"date" : "2013-07-25",
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"author" : "emil",
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"author" : "fabio",
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"title" : "The incompetent Bacillus subtilis saga:bacillus medium begins",
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"title" : " the blue ligation: the never ending story !",
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"content" : "<html>Firstable I did an inoculum of Neb10&beta; in order to make them competent the next day.I started to prepare the media needed for the transformation of Bacillus according to LMU munich protocol but due to the failure of this protocol I won't report the protocol.</html>",
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"content" : "<html> today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/c/ce/Tn-2013Eee.jpg\" width=\"450px\" /></center></html>}}<html><br>As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!</html>",
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"tags" : "Bacillus subtilis"
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"tags" : " YF1_FixJ - FixK2"
}
}

Revision as of 08:43, 3 October 2013

{ "date" : "2013-07-25", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : " today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula!

gel

As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!", "tags" : " YF1_FixJ - FixK2" }

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