Team:UNITN-Trento/Notebook/Labposts/07/06
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Revision as of 09:41, 3 October 2013
{ "date" : "2013-07-02", "author" : "emil", "title" : "Purification of the digestion products (K823026, K823024, E0840) ligation of the GFP in the 2 backbones and transformation of the ligation products ", "content" : "This morning I added 1µl of DPN1 to the GFP ligation and 1µl of SAP(alkaline phosphatase) to the 2 backbones.After 1h 30 min I have stopped the reaction by putting the reaction tubes at 80 C°.Afterwards I have purified the products following the purification protocol.Then I quantified the products with the following results:
Quantification
Then I performed the ligation(1:1,1:3,CTRL) of the GFP with the 2 backbone individually and following the ligation protocol.Finally I transformed 10µl of the ligation protocol in Neb10β and plate them on ampicillin LB agar following the transformation protocol.",
"tags" : "E0840-K823026-K823024"
}
Sample | Quantification |
---|---|
BBa_E0840 | 15.8ng/µl |
BBa_K823026 | 37.6ng/µl |
BBa_K823024 | 36.4ng/µl |