Team:UNITN-Trento/Notebook/Labposts/08/08
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{ | { | ||
- | "date" : "2013-08- | + | "date" : "2013-08-03", |
- | "author" : " | + | "author" : "gabriele", |
- | "title" : " | + | "title" : "Wintergreen with pLac-SAMsynthetase: Ep. II", |
- | "content" : "<html> | + | "content" : "<html>I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/µl) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!! </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>13A</td></tr><tr><td>13B</td></tr><tr><td>13C</td></tr><tr><td><i>Empty</i></td></tr><tr><td>14A</td></tr><tr><td>14B</td></tr><tr><td>14C</td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/f/f7/Tn-20130803_SAMinWGREEN_check_2.jpg\" /></html>}}<html>Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Screening Gel|<html><table><tr><td>Loading scheme</td></tr><tr><td>1kb ladder</td></tr><tr><td>13A</td></tr><tr><td>Ctrl</td></tr><tr><td><i>14B</i></td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/7/76/Tn-20130803_SAMinWGREEN_check_3.jpg\" /></html>}}<html></html>", |
- | "tags" : " | + | "tags" : "Wintergreen-SAMsynthetase-pLac" |
} | } |
Latest revision as of 09:43, 3 October 2013
{ "date" : "2013-08-03", "author" : "gabriele", "title" : "Wintergreen with pLac-SAMsynthetase: Ep. II", "content" : "I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/µl) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!!
Screening Gel
Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!Loading scheme |
13A |
13B |
13C |
Empty |
14A |
14B |
14C |
1kb ladder |
Screening Gel
",
"tags" : "Wintergreen-SAMsynthetase-pLac"
}
Loading scheme |
1kb ladder |
13A |
Ctrl |
14B |
1kb ladder |