Team:UNITN-Trento/Notebook/Labposts/08/08
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Latest revision as of 09:43, 3 October 2013
{ "date" : "2013-08-03", "author" : "gabriele", "title" : "Wintergreen with pLac-SAMsynthetase: Ep. II", "content" : "I miniprepped the six inocula from yesterday (great yields, minimum is 249.9 ng/µl) and screened them with EcoRI-HF and PstI-HF. One sample was positive! YEAH!!!
Screening Gel
Being completely honest I admit that from the first gel the result was not clear, so I performed a second electroforesis run with a ctrl (wintergreen alone, digested with EcoRI-HF and PstI-HF). In this last screen it is clear how 13A is positive!Loading scheme |
13A |
13B |
13C |
Empty |
14A |
14B |
14C |
1kb ladder |
Screening Gel
",
"tags" : "Wintergreen-SAMsynthetase-pLac"
}
Loading scheme |
1kb ladder |
13A |
Ctrl |
14B |
1kb ladder |