Team:UNITN-Trento/Notebook/Labposts/07/33

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Latest revision as of 10:40, 3 October 2013

{"date" : "2013-07-15","author" : "thomas","title" : "Ethylene production kinetic assay (third and last trial)","content" : "Since I was not satisfied with the curve obtained during the last experiment, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.

Plot

Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.","tags" : "EFE"}

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+{"date" : "2013-07-15","author" : "thomas","title" : "Ethylene production kinetic assay (third and last trial)","content" : "<html>Since I was not satisfied with the curve obtained during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-08-thomas\">last experiment</a>, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/00/Tn-2013_kinetic_EFE_plot-2.png\" style =\"width: 450px\"></center></html>}}<html>Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.</html>","tags" : "EFE"}
-	"date" : "2013-07-29",
-	"author" : "emil",
-	"title" : "The incompetent Bacillus saga:the final transformation",
-	"content" : "<html>Finally we have decided to try to transform PXyl+GFP that is an integrative vector for the thr locus.I have digested 1&micro;g PXyl with the enzyme Sca1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> standard protocol for the double digestion </a>and exploiting the specific buffer included in the kit from Biolabs.I have previously grown B. subtilis in the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation'>medium from Groeningen </a> o.n.,this morning I diluted the Bacillus(1:100) in the same medium then when it reached the O.D. of 1.1 I added 1&micro;l of DNA and I let grow for two hours.After that I plated on spectinomicin added LB agar(stock solution 100mg/ml).</html>",
-	"tags" : "B.subtilis-PXyl"
-}