From 2013.igem.org
(Difference between revisions)
|
|
| (2 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | { | + | {"date" : "2013-07- 23","author" : "emil","title" : " The incompetent Bacillus subtilis saga: The LB transformation and amplification of pSpac +GFP","content" : "<html>I have transformed 3 groups of cells with 500 ng of pSpac+pLac+RFP,I grew Bacillus in LB and when it reached OD of 1.1 I added the DNA to 400µl of cells,I let grow for 1 hour and then I plated the cell on Kanamicin plates. Afterwards I transformed Neb10& beta; with 100 ng of K823026+ E0840 following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols# Competent-cells-transformation\"> transformation protocol</a>.</html>","tags" : " Bacillus subtilis-pSpac +GFP"} |
| - | "date" : "2013-07-10",
| + | |
| - | "author" : "emil",
| + | |
| - | "title" : " Purification of Inocula(09/7): the final countdown",
| + | |
| - | "content" : "<html>I (yes I'm egocentric) did the miniprep(wath a new thing!) of the inocula(I didn't purified the 1:2 due to their despicable red color) previously done(the last I hope) following(is always the same but I have to write it anyway)the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">miniprep protocol</a>(OOOH!that's incredible!).These are(obviously)the results of the quantification:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantities</th></tr><tr><td>BBa_K823026+BBa_E0840(1 :1) </td><td> 421. 3ng/µl</td></tr><tr><td>BBa_K823026+BBa_E0840(1 :1) </td><td> 438.6ng/µl </td></tr>tr><td>BBa_K823026+BBa_E0840(1 :3) </td><td> 356. 7ng/& micro; l</td></tr><tr><td>BBa_K823026+ BBa_E0840(1:3) </td><td> 415.2ng/µl</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td> 401.2ng/µl</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td> 365ng/µl</td></tr></table></center></html>}}<html>Afterwards I screened them(800 ng for each sample) following the <a href =\"https://2013.igem.org/Team:UNITN-Trento/Protocols# Digestion\"> digestion protocol</a> so I have obtained these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel order|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Well</th></tr> <tr><td>ladder </td><td>1</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>2</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>3</td></tr>tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>4</td></tr><tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>5</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td>6</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td>7</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|image|<html><center><img src=\"https://static. igem.org/mediawiki/2013/1/15/Tn-2013_DSC0161.JPG\" width=\"450px\"></html>}}<html>As we can see in the last 4 samples the lower band is clearly at 1000 bp then the GFP is probably present, the succesfull samples were sent to be sequenced!</html>", | + | |
| - | "tags" : "pSpac-PXyl-GFP"
| + | |
| - | } | + | |
Latest revision as of 10:45, 3 October 2013
{"date" : "2013-07-23","author" : "emil","title" : "The incompetent Bacillus subtilis saga: The LB transformation and amplification of pSpac +GFP","content" : "I have transformed 3 groups of cells with 500 ng of pSpac+pLac+RFP,I grew Bacillus in LB and when it reached OD of 1.1 I added the DNA to 400µl of cells,I let grow for 1 hour and then I plated the cell on Kanamicin plates.Afterwards I transformed Neb10β with 100 ng of K823026+E0840 following the transformation protocol.","tags" : "Bacillus subtilis-pSpac+GFP"}
"
