Team:UNIK Copenhagen/Signe Notebook

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<h1> Notebook </h1>
 
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<p>Get our Nootebook in a printable version <a href="#"> here </a>.</p>
 
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<h1>Week 1 - July 8<sup>th</sup>-14<sup>th</sup> </h1>
 
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<p>
 
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<u>Culturing of magnetotactic bacteria (MTBs)</u> <br>
 
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Making base media for growing magnetotactic bacteria. This media was also used to make agar plates.
 
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MS-1 inoculated in liquid base medium. Falcon tubes were flushed with the nitrogen before use and incubated at 28 degrees.
 
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Plates were kept in a  closed container with an Oxoid AnaeroGen pad.<br><br>
 
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<u>Assembly of constructs</u> <br>
 
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Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates.
 
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Primers for MamC (MS-1 strain) was ordered.
 
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</p>
 
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</div>
 
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<div class="right_page">
 
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<p>Team photo in the park at Frederiksberg Campus </p>
 
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<a href="https://static.igem.org/mediawiki/2013/2/25/UNIK_Copenhagen_slide1.jpeg" target="_blank">
 
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<img src="https://static.igem.org/mediawiki/2013/2/25/UNIK_Copenhagen_slide1.jpeg" width="350">
 
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</a>
 
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<div id="content2" class="content">
 
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<div class="left_page">
 
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<h1>Week 2</h1>
 
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<p><u>Assembly of constructs</u>
 
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Overnight liquid cultures of pBBR1MCS-2 and eGFP plasmid (Adam Takos) <br>
 
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<br>
 
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Touchdown PCR amplification of MamC from MS-1 gDNA (both Phusion and Hotmaster) and of eGFP (only Hotmaster).
 
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<br>
 
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<a href="https://static.igem.org/mediawiki/2013/3/35/UNIK_Copenhagen_Notebook_ref_12.04.jpg" target="_blank">
 
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<img src="https://static.igem.org/mediawiki/2013/3/35/UNIK_Copenhagen_Notebook_ref_12.04.jpg" height="200">
 
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</a>
 
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<br>
 
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Gel purification of bands from PCR amplification and subsequent nanodrop. Due to poor yield from this extraction gradient PCR was performed (56-68 degrees).
 
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Picture 12.20
 
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Traditional cloning of eGFP fragments into pDRIVE.
 
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Transformation of eFbFP-pEX-A (synthesized gene, Eurofins) and eGFP-pDRIVE.
 
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</p>
 
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</div>
 
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<div class="right_page">
 
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<p>Gel purification of bands from PCR amplification and subsequent nanodrop. Due to poor yield from this extraction gradient PCR was performed (56-68 degrees). <br><br>
 
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Picture 12.20
 
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<br><br>
 
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Traditional cloning of eGFP fragments into pDRIVE.
 
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Transformation of eFbFP-pEX-A (synthesized gene, Eurofins) and eGFP-pDRIVE. </p>
 
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</div>
 
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</div>
 
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<div id="content3" class="content">
 
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<div class="left_page">
 
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<h1>Week 3</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content4" class="content">
 
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<div class="left_page">
 
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<h1>Week 4</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content5" class="content">
 
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<div class="left_page">
 
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<h1>Week 5</h1>
 
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<p>This week the team . . .</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content6" class="content">
 
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<div class="left_page">
 
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<h1>Week 6</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content7" class="content">
 
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<div class="left_page">
 
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<h1>Week 7</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content8" class="content">
 
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<div class="left_page">
 
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<h1>Week 8</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content9" class="content">
 
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<div class="left_page">
 
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<h1>Week 9</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content10" class="content">
 
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<div class="left_page">
 
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<h1>Week 10</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content11" class="content">
 
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<div class="left_page">
 
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<h1>Week 11</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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</div>
 
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<div id="content12" class="content">
 
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<div class="left_page">
 
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<h1>Week 12</h1>
 
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<p>This week the team...</p>
 
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</div>
 
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<div class="right_page">
 
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<p> more text on this page!</p>
 
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</div>
 
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Latest revision as of 15:30, 4 October 2013