Team:Kent/Attributions

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!align="center"|[[Team:Kent/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:Kent/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:Kent/Notebook|Notebook]]
!align="center"|[[Team:Kent/Notebook|Notebook]]
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!align="center"|[[Team:Kent/Attributions|Attributions]]
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!align="center"|[[Team:Kent/Attributions| Attributions]]
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<big><ins>'''What we did.'''</ins></big>
<big><ins>'''What we did.'''</ins></big>
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In our project, we managed to create two novel biobricks containing the NO sensing promoter [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1153000 orV] and the other containing the [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1153001 nrfAgene though unfortunately we ran out of time to create the unified biobrick which contained both of these parts. However these parts will form good building blocks for potential future projects either at Kent or other Universities for testing for the presence of NOx or its conversion to Ammonia. We hope that our project has stimulated interest in alternatives to the current methods of NOx removal from the environment.
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In our project, we managed to create two novel biobricks containing the NO sensing promoter ''norV'' and the other containing the ''nrfA'' gene. These parts will form building blocks for potential future projects for testing for the presence of NOx or its conversion to Ammonia. We hope that our project has stimulated interest in alternatives to the current methods of NOx removal from the environment.
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We would like to thank those whose research we based this project on, the iGEM foundation, University of Kent and our project supervisors for giving us the chance to undertake this project.
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Our project is based on the research on ''norV'' and the ''nrfA'' genes reported in:
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Clarke, TA., Mills, PC. et al (2008). Escherichia coli cytochrome c nitrite reductase N''Italic text''rfA.. Methods in Enzymology. 437: 63-77. * Bush M, Ghosh T, Tucker N, Zhang X, Dixon R: Transcriptional regulation by the dedicated NO sensor, NorR: a route towards NO detoxification; Biochem. Soc. Trans. (2011) 39: 289–293; doi:10.1042/BST0390289
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Bush M, Ghosh T, Tucker N, Zhang X, Dixon R: Transcriptional regulation by the dedicated NO sensor, NorR: a route towards NO detoxification; Biochem. Soc. Trans. (2011) 39: 289–293; doi:10.1042/BST0390289
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Tucker N, Ghosh T, Bush M, Zhang X, Dixon R: Essential roles of here enhancer sites in σ54 – dependent transcription by the nitric oxide sensing regulatory protein NorR; Nucl. Acids Res. (2010) 38(4): 1182 – 1194; doi: 10.1093/nar/gkp1065.
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We would like to thank the iGEM foundation, University of Kent and our project supervisors for giving us the opportunity to undertake this project.

Latest revision as of 17:50, 4 October 2013

Home Official Team Profile Team Project Parts Submitted to the Registry Notebook Attributions

What we did.

In our project, we managed to create two novel biobricks containing the NO sensing promoter norV and the other containing the nrfA gene. These parts will form building blocks for potential future projects for testing for the presence of NOx or its conversion to Ammonia. We hope that our project has stimulated interest in alternatives to the current methods of NOx removal from the environment.

Our project is based on the research on norV and the nrfA genes reported in:

Clarke, TA., Mills, PC. et al (2008). Escherichia coli cytochrome c nitrite reductase NItalic textrfA.. Methods in Enzymology. 437: 63-77. * Bush M, Ghosh T, Tucker N, Zhang X, Dixon R: Transcriptional regulation by the dedicated NO sensor, NorR: a route towards NO detoxification; Biochem. Soc. Trans. (2011) 39: 289–293; doi:10.1042/BST0390289

Bush M, Ghosh T, Tucker N, Zhang X, Dixon R: Transcriptional regulation by the dedicated NO sensor, NorR: a route towards NO detoxification; Biochem. Soc. Trans. (2011) 39: 289–293; doi:10.1042/BST0390289

Tucker N, Ghosh T, Bush M, Zhang X, Dixon R: Essential roles of here enhancer sites in σ54 – dependent transcription by the nitric oxide sensing regulatory protein NorR; Nucl. Acids Res. (2010) 38(4): 1182 – 1194; doi: 10.1093/nar/gkp1065.

We would like to thank the iGEM foundation, University of Kent and our project supervisors for giving us the opportunity to undertake this project.