Team:XMU-China/Content parts

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<tr><td>Fig.7-6 AGE confirmation of plasmid pSB4K5-ndh construction
<tr><td>Fig.7-6 AGE confirmation of plasmid pSB4K5-ndh construction
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Revision as of 17:10, 26 October 2013

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Parts

Table 1. The Bio-bricks used in the study

Part

Backbone

Type

Location

Size (bp)

Description

part

backbone

BBa_K546000

pSB1C3

Signaling

2013-P1-12D

1964

2070

Lux pL controlled LuxR with lux pR autoinducing LuxI (lva tag)- AHL.

BBa_I763020

pSB1C3

Intermediate

RBS-GFP-TT

2013-P3-11H

914

2070

RBS-GFP (+LVA Tag)-Terminators

BBa_F2621

pSB1A2

Signaling

2013-P2-21F

1158

2079

3OC6HSL Receiver Device

BBa_K546001

pSB1C3

Device

2013-P1-12F

2135

2070

AIIA

AHL Reporter and Quencher

BBa_J04450

pSB4K5

Reporter

2013-P5-5G

1069

3419

RFP Coding Device

BBa_J04450

pSB3T5

Reporter

2013-P5-7C

1069

3241

RFP Coding Device

BBa_J23022

BBa_J23006

Composite

2013-P5-3H

234

2356

[key3d][TT]


Favorite XMU-China 2013 iGEM Team Parts

W/N Name Type Description Designer Length
W BBa_K1036000 Composite lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II Shengquan Zeng
Zhaopeng Cheng		 2687
W BBa_K1036001 Coding the ndh gene coding for respiratory NADH dehydrogenase II Shengquan Zeng
Zhaopeng Cheng		 1366
W BBa_K1036003 Composite lux pL controlled luxR with lux pR controlled gfp (LVA-tag) Xin Guo
Xi Xi		 4052
W BBa_K1036006 Composite lux pL controlled luxR with lux pR controlled sfgfp (with LVA-tag) Zhaopeng Cheng
Xiyu Wu		 4046


XMU-China 2013 iGEM Team Parts Sandbox

W/N Name Type Description Designer Length
W BBa_K1036002 Composite gfp (with LVA-tag) under plux R control Xin Guo
Xi Xi		 2866
W BBa_K1036004 Reporter sfgfp with LVA-tag Zhaopeng Cheng
Xiyu Wu		 753
W BBa_K1036005 Composite sfgfp (with LVA-tag) under plux R control Zhaopeng Cheng
Xiyu Wu		 2880


Agarose Gel Electrophoresis Confirmation for all Plasmids

pSB1C3-gfp-luxI

This plasmid consists of two parts of target genes: gfp and luxI, and both of them are regulated by quorum sensing promoter. High copy number. (Fig.7-1)

Fig.7-1 AGE confirmation of plasmid pSB1C3-gfp-luxI construction

pSB1C3-sfgfp-luxI

This plasmid consists of two parts of target genes: sfgfp and luxI, and both of them are regulated by quorum sensing promoter. sfGFP gene is included into this plasmid as a parallel experiment group of pSB1C3-gfp-luxI. High copy number. (Fig.7-2)

Fig.7-2 AGE confirmation of plasmid pSB1C3-sfgfp-luxI construction

p3H-GFP-luxI

This plasmid consists of two parts of target genes: gfp and luxI, and both of them are regulated by quorum sensing promoter. Middle copy number. (Fig.7-3)

Fig.7-3 AGE of p3H-gfp construction

p3H-sfGFP-luxI

This plasmid consists of two parts of target genes: sfgfp and luxI, and both of them are regulated by quorum sensing promoter. sfGFP gene is included into this plasmid as a parallel experiment group of pSB1C3-gfp-luxI. Middle copy number. (Fig.7-4)

Fig.7-4 AGE of p3H-sfgfp construction

pSB3T5-aiiA

This plasmid has one target gene aiiA under the regulation of quorum sensing promoter. Middle copy number. (Fig. 7-5)

Fig.7-5 AGE confirmation of plasmid pSB3T5-aiiA construction

pSB4K5-ndh

This plasmid has one target gene aiiA under the regulation of quorum sensing promoter. Low copy number. (Fig. 7-6)

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Fig.7-6 AGE confirmation of plasmid pSB4K5-ndh construction