Team:Michigan/Project

From 2013.igem.org

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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<h2>Project Description</h2>
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!align="center"|[[Team:Michigan|Home]]
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!align="center"|[[Team:Michigan/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Michigan Official Team Profile]
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!align="center"|[[Team:Michigan/Project|Project]]
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!align="center"|[[Team:Michigan/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Michigan/Modeling|Modeling]]
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!align="center"|[[Team:Michigan/Notebook|Notebook]]
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!align="center"|[[Team:Michigan/Safety|Safety]]
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!align="center"|[[Team:Michigan/Attributions|Attributions]]
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<embed width=100% height=800; src="https://static.igem.org/mediawiki/2013/4/4e/ProjectDescription.pdf"></embed>
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== '''Overall project''' ==
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Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)
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Recent studies have just started to explore the possibility of utilizing existing recombination systems that flip segments of DNA, to store information and perform computations. However, the only systems studied so far are not completely unidirectonal in their ability to flip a segment of DNA. Other systems studied to date have consisted of one unidirectional recombinase capable of flipping a segment of DNA in only one direction, but have lacked a complementary unidrectional recombinase to flip the DNA back in only the opposite direction. Instead, previous systems have relied on “recombination directionality factors”, which when complexed with the unidirectional recombinase, reverse the direction in which it flips the DNA segment. The fim system from E. coli, has been shown to contain 2 unidirectional recombinases, hbif and fime, which flip a promoter containing segment of DNA. Our project seeks to engineer the fim switch by replacing the native promoter with another promoter. We plan to demonstrate that it can function as a reliable and efficient biological transistor, or “transcriptor”. Beyond storing information and performing basic computations, the system would serve as a very useful, tightly controlled switch.
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 03:33, 28 October 2013

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Project Description