Team:USTC CHINA/Modeling/

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the best growing condition for Bacillus subtilis WB800N
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<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a></div></div>
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<h1>Modeling on the suicide mechanism of the reporter system</h1>
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<p>To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine.
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Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.</p>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">read more about Kill Switch</a>
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To ensure the expression of our T-vaccine, we do some experiments to find out the best growing condition for Bacillus subtilis WB800N, we finally choose five independent factors: temperature, inoculation time, peptone, yeast extract and NaCl. The following table displays their levels:
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<h1>The best growing condition for <i>B.subtilis</i> WB800N</h1>
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图无法导出
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To ensure the expression of our T-vaccine, we do some experiments to find out the best growing condition for <i>B.subtilis</i> WB800N, we finally choose five independent factors: temperature, inoculation time, peptone, yeast extract and NaCl. The following table displays their levels:
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Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for Bacillus subtilis.
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  <th align="left">Factor</th>
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  <th align="right">Low</th>
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  <th align="right">High</th>
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  <td align="left">Temperature</td>
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  <td align="right">25℃</td>
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  <td align="right">35℃</td>
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  <td align="left">Time</td>
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  <td align="right">12h</td>
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  <td align="right">24h</td>
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  <td align="left">Peptone</td>
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  <td align="right">5</td>
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  <td align="right">15</td>
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  <th align="left">Yeast Extract</th>
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  <th align="right">2.5</th>
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  <th align="right">7.5</th>
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  <th align="left">NaCl</th>
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  <th align="right">5</th>
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  <th align="right">15</th>
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</table>
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<div>
 
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<h1>Modeling on the suicide mechanism of the reporter system</h1>
 
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To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine.
 
-
Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.
 
</div>
</div>
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<br><br>
<div>
<div>
 +
Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for <i>B.subtilis</i>.
 +
<br>
 +
<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">read more about <i>B.Subtilis Culture</i></a>
</div>
</div>
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<br><br>
<div>
<div>
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<h1>Designs of Immune Experiments</h1>
 +
<p>Our mice experiment has primarily proven the validity of our project. However, just like most scientific immune experiments on animals, the aim of our mice experiment was verification instead of exploring the optimal conditions for the production of our vaccine. In fact, fewer optimization experiments have been done by pure scientific researches, as most scientists care about facts and theories only, whereas exploring the optimal conditions is often viewed as the task of pharmaceutical factories. Yet since igem itself frequently involves industrial fields, which make igem seems like more an engineered competition than a science competition sometimes. </p>
 +
<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">read more about Designs of Immune Experiments</a>
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<div class="port-sidebar-border"><h>Modeling</h></div>
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<div class="clear"></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs Of Immune Experiments</a></div>
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</div></div></div>
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Latest revision as of 12:39, 28 October 2013

Modeling on the suicide mechanism of the reporter system

To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine. Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.

read more about Kill Switch


The best growing condition for B.subtilis WB800N

To ensure the expression of our T-vaccine, we do some experiments to find out the best growing condition for B.subtilis WB800N, we finally choose five independent factors: temperature, inoculation time, peptone, yeast extract and NaCl. The following table displays their levels:
Factor Low High
Temperature 25℃ 35℃
Time 12h 24h
Peptone 5 15
Yeast Extract 2.5 7.5
NaCl 5 15


Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for B.subtilis.
read more about B.Subtilis Culture


Designs of Immune Experiments

Our mice experiment has primarily proven the validity of our project. However, just like most scientific immune experiments on animals, the aim of our mice experiment was verification instead of exploring the optimal conditions for the production of our vaccine. In fact, fewer optimization experiments have been done by pure scientific researches, as most scientists care about facts and theories only, whereas exploring the optimal conditions is often viewed as the task of pharmaceutical factories. Yet since igem itself frequently involves industrial fields, which make igem seems like more an engineered competition than a science competition sometimes.

read more about Designs of Immune Experiments
Modeling

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