"
Team:DTU-Denmark/Notebook/6 August 2013
From 2013.igem.org
(Difference between revisions)
(→Results) |
(→Procedure) |
||
| Line 12: | Line 12: | ||
==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | |||
| + | ===PCR=== | ||
| + | |||
| + | lots of PCR done | ||
| + | |||
| + | ===gel purification=== | ||
| + | |||
| + | of the Nir1 fragment extraced from P. aeruginosa | ||
| + | |||
| + | ===plasmid miniprep=== | ||
| + | |||
| + | HAO in pZA21 from USER cloning was purified for restriction analysis | ||
==Results== | ==Results== | ||
Revision as of 15:01, 6 August 2013
6 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Kristian, Gosia, Natalia, Henrike
Procedure
PCR
lots of PCR done
gel purification
of the Nir1 fragment extraced from P. aeruginosa
plasmid miniprep
HAO in pZA21 from USER cloning was purified for restriction analysis
Results
1% agarose gel
- 1 kb ladder
- Nir1 col. T
- Nir1 col. T
- Nir2 col. T
- Nir2 col. T
- Nir1 frag. T
- Nir1 frag. T
- Nir1 col. X7
- Nir1 col. X7
- neg
- Hi
- Hi
- DMSO
- DMSO
- Nir1
- Nir1
- Nir2
- Nir2
- 1 kb ladder
1% agarose gel
- 1 kb ladder
- NirW
- NirW
- araBAD biobrick K808000
- araBAD biobrick K808000
- SPL in pZA21 containing RFP
- SPL in pZA21 containing RFP
- reference promoter in pZA21 containing RFP
- reference promoter in pZA21 containing RFP
- neg
- neg
- 1 kb ladder
(pic small gel)
Conclusion
Navigate to the Previous or the Next Entry
