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Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period10/Dailylog
From 2013.igem.org
(Created page with "{{TeamBYUProvo}} <br> {| width="100%" | colspan="3" | <font color="#333399" size="5" font face="Calibri"> : '''Phage Purification July - August Notebook: August 1 - August 16...") |
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- Performed the spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. | - Performed the spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. | ||
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- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. | - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. | ||
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| - | - | + | - Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team. |
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Revision as of 21:05, 7 August 2013
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8/2/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/5/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.
8/7/13 - Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team.
8/4/13 - Started E coli liquid culture over night
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Experiment 1
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