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Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period10/Dailylog
From 2013.igem.org
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| - | - | + | - We performed dialysis on T7 mutated phage from the cesium chloride gradient on 8.7. Because the gradient only showed one band, we decided to not do an EM as we most likely had mutant phage mixed in with wild type. We discussed a gradient to run in the future that will be able to further identify where the phage band. We decide to run a CsCl gradient with concentrations of 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.6. We hope to be able to identify exactly where the phage bands so that we can make small variations in the gradient at that point. This will allow us to separate mutant phage from wild type. |
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Revision as of 20:04, 12 August 2013
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8/2/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/5/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.
8/7/13 - Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team.
8/9/13 - We performed dialysis on T7 mutated phage from the cesium chloride gradient on 8.7. Because the gradient only showed one band, we decided to not do an EM as we most likely had mutant phage mixed in with wild type. We discussed a gradient to run in the future that will be able to further identify where the phage band. We decide to run a CsCl gradient with concentrations of 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.6. We hope to be able to identify exactly where the phage bands so that we can make small variations in the gradient at that point. This will allow us to separate mutant phage from wild type.
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Experiment 1
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