Team:ETH Zurich/Experiments

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Revision as of 14:20, 20 August 2013

Header2.png
80px-Eth igem logo.png

Tranformation and cloning constructs


Sender cells Receiver cells
AHL expressing system Fluorescent reporter system Enzyme reaction-based reporter system
Pcons-LuxI 1 (Strong) Plac-LuxR-Plux-GFP Plac-LuxR-Plux-LacZ
Pcons-LuxI 2 Plac-LuxR-Plux-YFP Plac-LuxR-Plux-Aes
Pcons-LuxI 3 Plac-LuxR-Plux-CFP Plac-LuxR-Plux-GusA
Pcons-LuxI 4 (Weak) Plac-LuxR-Plux-PhoA
Plac-LuxR-Plux-NagZ
Biobrick Registry part
LuxI K805016
Pcons 1 (Strong) J23100
Pcons 2 J23110
Pcons 3 J23114
Pcons 4 (Weak) J23118
Mutagenized promoter 1
( low [AHL])
Optimized Biobrick !
Mutagenized promoter 2
( middle [AHL])
Optimized Biobrick !
Mutagenized promoter 3
( high [AHL])
Optimized Biobrick !
pLuxR R0062
Plux
Plac
GFP E0840
YFP E0030
CFP E0020
LacZ I732006
GusA New Biobrick !
PhoA New Biobrick !
Aes New Biobrick !
NagZ New Biobrick !












Here we will paste some circuit designs


Enzyme-substrate reactions


We have cloned fluorescent receiver systems as backup for our circuit in case the hydrolase reaction do not work properly.
The enzyme substrate reactions take less than 5 minutes and are visible by eye.

Our minesweeper become better and better so keep on track for updates !

Hydrolase Complementary substrate / IUPAC name Visible color
LacZ Beta-Galactosidase X-Gal 5-Bromo-4chloro-3-indolyl-beta-galactopyranoside Blue
LacZ Beta-Galactosidase Green-beta-D-Gal N-Methyl-3-indolyl-beta-D_galactopyranoside Green
GusA Beta-glucuronidase Magenta glucuronide 6-chloro-3-indolyl-beta-D-glucuronide-cycloheylammonium salt Red
PhoA Alkaline phosphatase pNPP 4-Nitrophenylphosphatedi(tris) salt Yellow
Aes Carboxyl esterase Magenta butyrate 5-bromo-6-chloro-3-indoxyl butyrate Magenta
NagZ Glycoside hydrolase X-glucosaminide X-Glunac 5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide Blue