29/08/13
From 2013.igem.org
(Difference between revisions)
(Created page with "==Make 2% agarose gel== ==Run Tod operon ligation genes on the gel== ==Isolate the DNA from gel== *DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol") |
(→Run Tod operon ligation genes on the gel) |
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==Make 2% agarose gel== | ==Make 2% agarose gel== | ||
==Run Tod operon ligation genes on the gel== | ==Run Tod operon ligation genes on the gel== | ||
+ | *1.5h at 70V | ||
+ | |||
==Isolate the DNA from gel== | ==Isolate the DNA from gel== | ||
*DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol | *DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol |
Revision as of 14:00, 29 August 2013
Make 2% agarose gel
Run Tod operon ligation genes on the gel
- 1.5h at 70V
Isolate the DNA from gel
- DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol