Exeter/7 August 2013

From 2013.igem.org

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Glycerol stocks
Glycerol stocks
*200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.  
*200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.  
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*Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow
*Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow
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We miniprepped:
 
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==We miniprepped:==
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* B0034 (RBS) AMP
 
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* K592010 (yellow) CAM
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* [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 B0034] (RBS) AMP
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* S05058 (RBS + yellow) CAM
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* [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM
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* K608002 (Promoter and RBS) CAM
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* [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM
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* RFP control CAM
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* [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (Promoter and RBS) CAM
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* [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
* 3 NEB (RFP and Terminator) AMP
* 3 NEB (RFP and Terminator) AMP
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* K592012 (Magenta) CAM
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* [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM
* NEB4 (RBS and cph8) AMP
* NEB4 (RBS and cph8) AMP
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* NEB1 (RBS and cph8) AMP
* NEB1 (RBS and cph8) AMP
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* B0015 (Terminator) CAM
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* [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM
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* K592018 (RBS + Cyan) CAM
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* [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + Cyan) CAM
== Adam and Tom ==
== Adam and Tom ==
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Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)
Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Latest revision as of 19:56, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Glycerol stocks

  • 200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.
  • Store at -80 degrees celsius
  • Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow


We miniprepped:

  • [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 B0034] (RBS) AMP
  • [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM
  • [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM
  • [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (Promoter and RBS) CAM
  • [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
  • 3 NEB (RFP and Terminator) AMP
  • [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM
  • NEB4 (RBS and cph8) AMP
  • NEB1 (RBS and cph8) AMP
  • [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM
  • [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + Cyan) CAM

Adam and Tom

Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli