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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
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| + | <h3>General protocols and <i>E. coli</i> protocols</h3> | ||
| + | <ul> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/sob">SOB medium</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/tae">TAE buffer</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/plates"></a>LB plates</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">Gelelectrophoresis</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">Chemo-competent <i>E. coli</i> cells</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"></a><i>E. coli</i> transformation</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/pgem-ligation">pGEM Ligaton - ligation for TA-cloning of PCR products</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/ligation">Ligation of digested parts and vectors</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/glycerol-stocks">Glycerol stocks</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/pcr"></a>PCR</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/3a-assembly"></a>3A-Assembly</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/preparative-restriction"></a>Preparative restriction digest</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/control-restriction"></a>Control restriction digest</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/mini-prep"></a>Mini-Prep</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/gelextraction"></a>Gelextraction</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li> | ||
| + | </ul> | ||
| + | |||
| + | <h3><i>Saccharomyces cerevisiae</i> specific protocols</h3> | ||
| + | <ul> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/ypd">YPD medium</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo"></a>Yeast transformation</li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/"></a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/"></a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/"></a></li> | ||
| + | </ul> | ||
Revision as of 21:20, 3 October 2013
Protocols
General protocols and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- Gelelectrophoresis
- Chemo-competent E. coli cells
- E. coli transformation
- pGEM Ligaton - ligation for TA-cloning of PCR products
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini-Prep
- Gelextraction
- Colony-PCR
Saccharomyces cerevisiae specific protocols
- YPD medium
- SC DropOut
- Yeast transformation
