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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
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<li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/colony-pcr">Colony-PCR</a></li> | ||
</ul> | </ul> | ||
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| + | <p>&nbps;</p> | ||
<h3><i>Saccharomyces cerevisiae</i> specific protocols</h3> | <h3><i>Saccharomyces cerevisiae</i> specific protocols</h3> | ||
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<li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/sc-dropout">SC DropOut</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/yeast-trafo">Yeast transformation</a></li> | ||
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</ul> | </ul> | ||
Revision as of 21:21, 3 October 2013
Protocols
General protocols and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- Gelelectrophoresis
- Chemo-competent E. coli cells
- E. coli transformation
- pGEM Ligaton - ligation for TA-cloning of PCR products
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini-Prep
- Gelextraction
- Colony-PCR
&nbps;
Saccharomyces cerevisiae specific protocols
