Template:Kyoto/Notebook/Aug 28

From 2013.igem.org

(Difference between revisions)
(Ligation)
(Ligation)
Line 47: Line 47:
|3||4||3.5||10.5
|3||4||3.5||10.5
|}
|}
-
*incubate overnight at 4 °C
+
*incubate one hour at 16 °C
</div>
</div>

Revision as of 05:57, 21 September 2013

Contents

Aug 28

Electrophoresis

No name

LaneSample
1100bp
2SasA
3RpaA
4RpaB
5PkaiBC
6PCR NC
7100bp

File:Igku xxxxxx.xxx

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
8/27 Plux173.81.951.45

Ligation

No name

stateVectorInserter
experiment8/71 DT-13.08.0
  • Samples were evaporeted used evaporator into about 3 µL.
sampleMilliQLigation Hightotal
343.510.5
  • incubate one hour at 16 °C

Gel Extraction

Colony PCR

Restriction Enzyme Digestion

Electrophoresis

Gel Extraction

Colony PCR

Liquid Culture

Ligation

Liquid Culture