Template:Kyoto/Notebook/Aug 29
From 2013.igem.org
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===Electrophoresis=== | ===Electrophoresis=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">No name</span> | ||
+ | {| class="wikitable" | ||
+ | !Lane||Sample | ||
+ | |- | ||
+ | |1||100bp ladder | ||
+ | |- | ||
+ | |2||8/21 RBS-lysis2-9(Colony PCR prodution) | ||
+ | |- | ||
+ | |3||8/21 RBS-lysis2-8(Colony PCR prodution) | ||
+ | |- | ||
+ | |4||8/21 RBS-lysis2-7(Colony PCR prodution) | ||
+ | |- | ||
+ | |5||8/21 RBS-lysis2-10(Colony PCR prodution) | ||
+ | |- | ||
+ | |6||8/21 RBS-lysis2-11(Colony PCR prodution) | ||
+ | |- | ||
+ | |7||8/21 RBS-lysis2-12(Colony PCR prodution) | ||
+ | |- | ||
+ | |8||8/21 RBS-lysis2-13(Colony PCR prodution) | ||
+ | |- | ||
+ | |9||8/21 RBS-lysis2-14(Colony PCR prodution) | ||
+ | |} | ||
+ | [[File:igku_xxxxxx.xxx]]<br> | ||
+ | </div> |
Revision as of 09:02, 21 September 2013
Aug 29
Colony PCR
Sample | base pair |
---|---|
8/27 Pcon-pT181 antisense-(1) | 542 |
8/26 pT181 attenuator-DT-(1) | 738 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 30s | 30cycles |
Electrophoresis
Lane | Sample |
---|---|
1 | 100bp ladder |
2 | 8/21 RBS-lysis2-9(Colony PCR prodution) |
3 | 8/21 RBS-lysis2-8(Colony PCR prodution) |
4 | 8/21 RBS-lysis2-7(Colony PCR prodution) |
5 | 8/21 RBS-lysis2-10(Colony PCR prodution) |
6 | 8/21 RBS-lysis2-11(Colony PCR prodution) |
7 | 8/21 RBS-lysis2-12(Colony PCR prodution) |
8 | 8/21 RBS-lysis2-13(Colony PCR prodution) |
9 | 8/21 RBS-lysis2-14(Colony PCR prodution) |