Template:Kyoto/Notebook/Aug 31

From 2013.igem.org

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|experiment||8/22 Plac(SpeI&PstI)||8.0||8/18 RBS-lacZα-DT||10||3.5
|experiment||8/22 Plac(SpeI&PstI)||8.0||8/18 RBS-lacZα-DT||10||3.5
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Samples were evaporeted used evaporator into about 7 µL
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Revision as of 13:18, 22 September 2013

Aug 31

Gel Extraction

No name

LaneDNAEnzyme
1100bp ladder--
2----
3RBS-lysis2-(9)EcoRI & SpeI
4
5----
6pSB1C3(1)EcoRI & SpeI
7
8----
9pSB1C3(1)XbaI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

LaneDNAEnzyme
1100bp ladder--
2tRNA-SpinachEcoRI & SpeI
3
4----
5pT181 attenuator-(2)EcoRI & SpeI
6
7pT181 attenuator-(2)XbaI & PstI
8
9----
10pT181 antisense-(2)EcoRI & SpeI
11

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

LaneDNAEnzyme
1100bp ladder--
2pT181 antisenseXbaRI & PstI
3
4----
5tetR aptamer 12_1R-(1)EcoRI & SpeI
6
Nameconcentration[µg/mL]260/280260/230
RBS-lysis2(EcoRI&SpeI)4.02.690.24
pSB1C3(EcoRI&SpeI)6.02.480.05
pSB1C3(XbaI&PstI)------
tRNA-Spinach------
pT181 attenuator-(2)(EcoRI&SpeI)------
pT181 anttenuator-(2)(SbaI&PstI)------
pT181 antisense-(2)(EcoRI&SpeI)------
pT181 antisense-(2)(XbaI&PstI)------
tetR aptamer 12_1R(EcoRI&SpeI)------

Ligation

Nakamoto and Hirano

stateVectorInserterLigation High ver.2
experiment8/30 pSB1C3(EcoRI&SpeI)6.68/30 tRNA-Spinach(EcoRI&SpeI)12.53.5
experiment8/30 pSB1C3(EcoRI&SpeI)6.68/30 pT181 antisense(EcoRI&SpeI)12.53.5
experiment8/30 pSB1C3(XbaI&Pst)12.58/30 pT181 attenuator(XbaI&PstI)253.5
experiment8/30 pSB1C3(XbaI&Pst)12.58/30 pT181 antisense(XbaI&PstI)253.5
experiment8/20 DT6.68/30 pT181 attenuator(2)12.53.5
experiment8/20 DT6.68/30 pT181 antisense12.53.5
experiment8/27 DT3.08/30 tRNA-Spinach(EcoRI&SpeI)12.53.5
experiment8/20 DT6.68/30 RBS-lysis212.53.5
experiment8/22 J23100(SpeI&PstI)--RBS-tetR-DT--3.5
experiment8/22 Plac(SpeI&PstI)8.08/18 RBS-lacZα-DT103.5

Samples were evaporeted used evaporator into about 7 µL