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Team:SDU-Denmark/Tour31
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| - | The biosynthesis of isoprenoids in bacteria works through the methylerythritol phosphate pathway (MEP pathway). The pathway is initiated with a condensation reaction between D-glyceraldehyde 3-phosphate and pyruvate to produce <span class="tooltipLink">DXP</span> <span class="tooltip">1-deoxy-D-xylulose-5-phosphate</span> and CO2 catalysed by <span class="tooltipLink">Dxs.</span> <span class="tooltip">1-deoxyxylulose-5-phosphate synthase</span> | + | The biosynthesis of isoprenoids in bacteria works through the methylerythritol phosphate pathway (MEP pathway). The pathway is initiated with a condensation reaction between D-glyceraldehyde 3-phosphate and pyruvate to produce <span class="tooltipLink">DXP</span> <span class="tooltip">1-deoxy-D-xylulose-5-phosphate</span> and CO2 catalysed by <span class="tooltipLink">Dxs.</span> <span class="tooltip">1-deoxyxylulose-5-phosphate synthase</span> |
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| + | DXP is reductively isomerized to <span class="tooltipLink">MEP</span> <span class="tooltip">2-C-methyl-D-erythritol-4-phosphate</span> by <span class="tooltipLink">Dxr</span> <span class="tooltip">1-deoxy-D-xylulose-5-phosphate reductoisomerase</span> in a reversible NADPH-dependent reaction and subsequently converted to <span class="tooltipLink">CDP-ME</span> <span class="tooltip">4-diphosphocytidyl-2-C-methyl-D-erythritol</span> in a CTP substrate inhibited reaction catalysed by <span class="tooltipLink">IspD.</span> <span class="tooltip">4-diphosphocytidyl-2-C-methyl-D-erythritol synthase</span> | ||
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Revision as of 13:56, 26 September 2013
Bacteriorganic Rubber
Specifications
Rewiring of the E. coli machinery
MEP pathway The biosynthesis of isoprenoids in bacteria works through the methylerythritol phosphate pathway (MEP pathway). The pathway is initiated with a condensation reaction between D-glyceraldehyde 3-phosphate and pyruvate to produce DXP 1-deoxy-D-xylulose-5-phosphate and CO2 catalysed by Dxs. 1-deoxyxylulose-5-phosphate synthase
DXP is reductively isomerized to MEP 2-C-methyl-D-erythritol-4-phosphate by Dxr 1-deoxy-D-xylulose-5-phosphate reductoisomerase in a reversible NADPH-dependent reaction and subsequently converted to CDP-ME 4-diphosphocytidyl-2-C-methyl-D-erythritol in a CTP substrate inhibited reaction catalysed by IspD. 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase
