Team:Marburg/Notebook:June
From 2013.igem.org
(Difference between revisions)
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<ul class="lig"> | <ul class="lig"> | ||
<li>Insert und plasmid were transformed using a 1:2 ratio. Samples were incubated ON at RT.</li> | <li>Insert und plasmid were transformed using a 1:2 ratio. Samples were incubated ON at RT.</li> | ||
- | <li>Ligation product were transformed into chemical competent DH5α cells.</li> | + | <li>Ligation product were transformed into chemical competent DH5α cells (30 min ice, 90 sec 42°C, 45 min 37°C + 900 µl LB).</li> |
<li>Transformed cells were spread out on LB Amp culture plates.</li> | <li>Transformed cells were spread out on LB Amp culture plates.</li> | ||
<li>Incubation ON at RT.</li> | <li>Incubation ON at RT.</li> | ||
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</p> | </p> | ||
<p> | <p> | ||
- | <span class="exp"> | + | <span class="exp">Expectations</span> |
<ul class="exp"> | <ul class="exp"> | ||
<li>Lane 1: ~ 6,000 bp for pSB1A3_486476315</li> | <li>Lane 1: ~ 6,000 bp for pSB1A3_486476315</li> |
Revision as of 20:30, 1 October 2013