Team:UNITN-Trento/Notebook/Labposts/07/15

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Revision as of 08:24, 3 October 2013

{ "date" : "2013-07-08", "author" : "emil", "title" : " Purification of Inocula (03/7)", "content" : "I verifyed the status of the last inocula(05/7) that resulted a bit strange.

Inocula

Unfortunately The day before were inoculate uncorrect plates(03/7) so I have purified them following the purification protocol these are the results of the quantification.

Quantification

Sample	Quantities
BBa_K823026+BBa_E0840(1:1) 1	438.8 ng/µl
BBa_K823026+BBa_E0840(1:1) 2	400.7 ng/µl
BBa_K823024+BBa_E0840(1:1) 3	343.6 ng/µl(the only succesful)

Afterwards I screened 800 ng of the samples following the screening protocol with EcoR1 HF and Pst1, these are the results of the gel:

Gel order

Sample	Well
Ladder 1kb Fermentas	1
BBa_K823026+BBa_E0840(1:1) a	3
BBa_K823026+BBa_E0840(1:1) b	4
BBa_K823024+BBa_E0840(1:1)	5

Gel image

As we can see only the third(024) sample shows the insert at the right size of 1000 bp(the lower band), then I did the inocula of the plates of 5/07(1:1,1:2,1:3) and of the old plate that gives right results to amplify 024(1:1).", "tags" : "K823024-K823026-E0840" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-07-18",
+	"date" : "2013-07-08",
-	"author" : "fabio-bruno",
+	"author" : "emil",
-	"title" : " blue and red light sensors!!",
+	"title" : " Purification of Inocula (03/7)",
-	"content" : "<html>  today we performed the miniprep protocol on our inocula. yealds are: <br>006 a: 169,3 ng/ul; <br>006 b: 204,4ng/ul; <br>016 a: 204,9 ng/ul; <br>016 b: 173,7 ng/ul; <br>030 a: 276ng/ul; <br>030 b: 375,8ng/ul; <br>082 a: 173,1ng/ul; <br>082 b: 225,9 ng/ul; <br>After that we screened 006b, 016a,082 and 030.<br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 1|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/3f/Tn-2013_a.jpg\" width=\"450px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 2|<html><center><img src=\"https://static.igem.org/mediawiki/2013/1/18/Tn.2013_B.jpg\" width=\"450px\" /></center></html>}}<html>As we can see from the gels, we didn’t confirm the presence of any of them!! Now we are going to do a second set of inocula!</html>",
+	"content" : "<html>I verifyed the status of the last inocula(05/7) that resulted a bit strange.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Inocula|<html><img src=\"\" width=\"450px\" /></html>}}<html>Unfortunately The day before were inoculate uncorrect plates(03/7) so I have purified them following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\"> purification protocol </a> these are the results of the quantification.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantities</th></tr><tr><td>BBa_K823026+BBa_E0840(1:1) 1</td><td>438.8 ng/&micro;l</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) 2</td><td>400.7 ng/&micro;l</td></tr><tr><td>BBa_K823024+BBa_E0840(1:1) 3</td><td>343.6 ng/&micro;l(the only succesful)</td></tr></table></center></html>}}<html>Afterwards I screened 800 ng of  the samples following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">screening protocol</a> with EcoR1 HF and Pst1, these are the results of the gel:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel order|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Well</th></tr><tr><td>Ladder 1kb Fermentas</td><td>1</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) a</td><td>3</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) b</td><td>4</td></tr><tr><td>BBa_K823024+BBa_E0840(1:1) </td><td>5</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><img src=\"\" width=\"450px\" /></html>}}<html>As we can see only the third(024) sample shows the insert at the right size of 1000 bp(the lower band), then I did the inocula of the plates of 5/07(1:1,1:2,1:3) and of the old plate that gives right results to amplify 024(1:1).</html>",
-	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
+	"tags" : "K823024-K823026-E0840"
 }