Team:UNITN-Trento/Notebook/Labposts/07/29

From 2013.igem.org

(Difference between revisions)
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{
{
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"date" : "2013-07-31",
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"date" : "2013-07-16",
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"author" : "fabio",
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"author" : "fabio-bruno",
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"title" : " the blue ligation: the never ending story !",
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"title" : " blue and red light sensors!!",
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"content" : "<html>Today massive miniprepping y’all!! This is a wonderful day because one of my miniprep seems toOk!! Sample number 3 from the 3rd ligation doesn’t show the band that correspond to 016 not ligated to the 006 promoter! Tomorrow I’ll need to verify by onetaq pcr.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/3a/Tn-2013_Fff.jpg\" width=\"450px\" /></center></html>}}<html></html>",
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"content" : "<html> the time has come to extract from the 2013 kit some other new parts: they will be needed to allow our divice to be activated by blue or red light!! Awesome, don’t you think? The parts are: <br>K592016: a blue sensor with its response regulator; k592006: a promoter induced by the blue regulator; <br>k519030: the device with the red sensor and necessary enzymes; r0082: the red promoter. <br>We after extracting them we transformed in neb5a and plated. </html>",
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"tags" : "YF1_FixJ-FixK2"
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"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
}
}

Revision as of 08:32, 3 October 2013

{ "date" : "2013-07-16", "author" : "fabio-bruno", "title" : " blue and red light sensors!!", "content" : " the time has come to extract from the 2013 kit some other new parts: they will be needed to allow our divice to be activated by blue or red light!! Awesome, don’t you think? The parts are:
K592016: a blue sensor with its response regulator; k592006: a promoter induced by the blue regulator;
k519030: the device with the red sensor and necessary enzymes; r0082: the red promoter.
We after extracting them we transformed in neb5a and plated. ", "tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR" }