Team:UNITN-Trento/Notebook/Labposts/07/30

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Revision as of 08:32, 3 October 2013

{ "date" : "2013-07-16", "author" : "gabriele", "title" : "A short day", "content" : "I added 1µl of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1µl of SAP to G2A_linear-pSB1C3 O/N digestion, and then incubated both samples at 37°C for 1.5h. Finally, I stocked both samples at -20°C because I would like to ligate them together with the short digestion that I am going to perform tomorrow.", "tags" : "SAMsynthetase" }

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 {
-	"date" : "2013-07-30",
+	"date" : "2013-07-16",
-	"author" : "fabio",
+	"author" : "gabriele",
-	"title" : " the blue ligation: the never ending story !",
+	"title" : "A short day",
-	"content" : "<html>  today unfortunately I miniprepped the last inocula (from ligation nr. 4) and screening didn’t verify the presence of any ligation. What a drag!! The only thing that remains to be done is to inoculate more and more colonies from previous plates: 6 from ligation 3 (plate 1:1) and 6 from ligation nr. 4 (plate 1:1).</html>",
+	"content" : "<html>I added 1&micro;l of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1&micro;l of SAP to G2A_linear-pSB1C3 O/N digestion, and then incubated both samples at 37&deg;C for 1.5h. Finally, I stocked both samples at -20&deg;C because I would like to ligate them together with the short digestion that I am going to perform tomorrow.</html>",
-	"tags" : " YF1_FixJ - FixK2"
+	"tags" : "SAMsynthetase"
 }