Team:UNITN-Trento/Notebook/Labposts/08/28

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Latest revision as of 09:10, 3 October 2013

{ "date" : "2013-08-12", "author" : "thomas-viola-michele", "title" : "Minipreps and screening", "content" : "Starting from the o/n cultures prepared yesterday, we performed a miniprep purification, obtaining these results:

Quantification Results

Sample	Concentration
S04617 + EFE ligation 1:2 sample 1	459 ng/µl
S04617 + EFE ligation 1:2 sample 2	603.2 ng/µl
S04617 + EFE ligation 1:2 sample 3	294.2 ng/µl
AraCpBAD - EFE ligation 1:1 sample 1	434.4 ng/µl
AraCpBAD - EFE ligation 1:1 sample 2	346.3 ng/µl
AraCpBAD - EFE ligation 1:2 sample 1	351.4 ng/µl
AraCpBAD - EFE ligation 1:2 sample 2	293.7 ng/µl

We proceeded then performing a screening digestion followed by an electrophoresis analysis. Kapa universal ladder was adopted.

Gel image

As you can see from the image, AraCpBAD + EFE (BBa_K1065002) part is confirmed for all the 3 samples (lane 1 to 3, 2426 bp). Differently, BBa_S04617 + EFE (BBa_K1265002) is not confirmed (lane 5 to 7, 2460 bp expected) since the band is at an heigh of about 1400 bp. Probably the destination vector BBa_S04617 was closed itself without incorporating EFE.", "tags" : "pFixK-cI-pLambda-EFE-AraCpBAD" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-15",
+	"date" : "2013-08-12",
-	"author" : "caterina",
+	"author" : "thomas-viola-michele",
-	"title" : "fail with honor",
+	"title" : "Minipreps and screening",
-	"content" : "<html>Starting from the o/n cultures prepared yesterday, I performed a miniprep purification and then, I proceeded then performing a screening digestion followed by an electrophoresis analysis (Kapa universal ladder was adopted).</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/8/87/Tn-2013_Fail_J_016%2Bs17.jpg\" /></center></html>}}<html> As you can see, I failed the ligation of s04617 in J61002 with k592016. </html>",
+	"content" : "<html>Starting from the o/n cultures prepared <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-08-11-thomas-viola\">yesterday</a>, we performed a miniprep purification, obtaining these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><table><tr><th>Sample</th><th>Concentration </th></tr><tr><td>S04617 + EFE ligation 1:2 sample 1</td><td>459 ng/&micro;l</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 2</td><td>603.2 ng/&micro;l</td></tr><tr><td>S04617 + EFE ligation 1:2 sample 3</td><td>294.2 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 1</td><td>434.4 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:1 sample 2</td><td>346.3 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 1</td><td>    351.4 ng/&micro;l</td></tr><tr><td>AraCpBAD - EFE ligation 1:2 sample 2</td><td>293.7 ng/&micro;l</td></tr></table></center></html>}}<html> We proceeded then performing a screening digestion followed by an electrophoresis analysis. Kapa universal ladder was adopted.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/02/Tn2013_GEL_aracpbad-EFE_S04617%2BEFE.jpg\"></center></html>}}<html> As you can see from the image, AraCpBAD + EFE (BBa_K1065002) part is confirmed for all the 3 samples (lane 1 to 3, 2426 bp). Differently, BBa_S04617 + EFE (BBa_K1265002) is not confirmed (lane 5 to 7, 2460 bp expected) since the band is at an heigh of about 1400 bp. Probably the destination vector BBa_S04617 was closed itself without incorporating EFE.</html>",
-	"tags" : "light receptor"
+	"tags" : "pFixK-cI-pLambda-EFE-AraCpBAD"
 }