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Team:SDU-Denmark/Tour30
From 2013.igem.org
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<h2>The production system</h2> | <h2>The production system</h2> | ||
<h4>The micro-road to rubber</h4> | <h4>The micro-road to rubber</h4> | ||
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<span class="intro">Natural rubber is a chain</span> of several isoprene molecules. The rubber tree has a <span class="tooltipLink">prenyltransferase</span> | <span class="intro">Natural rubber is a chain</span> of several isoprene molecules. The rubber tree has a <span class="tooltipLink">prenyltransferase</span> | ||
<span class="tooltip"> <span class="tooltipHeader">Prenyltransferase</span>HRT2</span> enzyme, which links molecules of <span class="tooltipLink">IPP</span> <span class="tooltip"><span class="tooltipHeader">IPP</span>Isopentenyl pyrophosphate</span> into the <span class="tooltipLink">isoprene chain</span> <span class="tooltip"><span class="tooltipHeader">Isoprene chain</span>natural rubber</span>. To start the extension of the chain, the prenyltransferase needs a <span class="tooltipLink">DMAPP</span> <span class="tooltip"><span class="tooltipHeader">DMAPP</span>Dimethylallyl pyrophosphate</span> molecule. Thus, in order to produce rubber, the bacteria needs the prenyltranferase, IPP, and DMAPP. | <span class="tooltip"> <span class="tooltipHeader">Prenyltransferase</span>HRT2</span> enzyme, which links molecules of <span class="tooltipLink">IPP</span> <span class="tooltip"><span class="tooltipHeader">IPP</span>Isopentenyl pyrophosphate</span> into the <span class="tooltipLink">isoprene chain</span> <span class="tooltip"><span class="tooltipHeader">Isoprene chain</span>natural rubber</span>. To start the extension of the chain, the prenyltransferase needs a <span class="tooltipLink">DMAPP</span> <span class="tooltip"><span class="tooltipHeader">DMAPP</span>Dimethylallyl pyrophosphate</span> molecule. Thus, in order to produce rubber, the bacteria needs the prenyltranferase, IPP, and DMAPP. | ||
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<a class="popupImg alignRight" style="width:450px" target="_blank" href="https://static.igem.org/mediawiki/2013/1/13/SDU2013_Smartart_1.png" title="The basic idea is to convert sugar (glucose) into natural rubber through the glycolysis to yield pyruvate and Glyceraldehyde-3-phosphate (GAP). These are further converted into Isopentenyl pyrophosphate (IPP) and Dimethylallyl pyrophosphate (DMAPP) through the MEP pathway. A DMAPP and several IPP molecules can then be linked together into natural rubber through the action of the prenyltransferase (HRT2) from the rubber tree."> | <a class="popupImg alignRight" style="width:450px" target="_blank" href="https://static.igem.org/mediawiki/2013/1/13/SDU2013_Smartart_1.png" title="The basic idea is to convert sugar (glucose) into natural rubber through the glycolysis to yield pyruvate and Glyceraldehyde-3-phosphate (GAP). These are further converted into Isopentenyl pyrophosphate (IPP) and Dimethylallyl pyrophosphate (DMAPP) through the MEP pathway. A DMAPP and several IPP molecules can then be linked together into natural rubber through the action of the prenyltransferase (HRT2) from the rubber tree."> | ||
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The basic idea is to convert sugar (glucose) into natural rubber through the glycolysis to yield pyruvate and GAP. These are further converted into IPP and DMAPP through the MEP pathway. A DMAPP and several IPP molecules can then be linked together into natural rubber through the action of the prenyltransferase (HRT2) from the rubber tree. | The basic idea is to convert sugar (glucose) into natural rubber through the glycolysis to yield pyruvate and GAP. These are further converted into IPP and DMAPP through the MEP pathway. A DMAPP and several IPP molecules can then be linked together into natural rubber through the action of the prenyltransferase (HRT2) from the rubber tree. | ||
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<span class="intro">IPP and DMAPP</span> are molecules naturally present in <span class="specialWord">E. coli</span>, produced by the bacteria through the <span class="tooltipLink">MEP pathway.</span> <span class="tooltip"><span class="tooltipHeader">MEP pathway</span>Methylerythritol phosphate pathway</span> The MEP pathway converts molecules of <span class="tooltipLink">GAP</span> <span class="tooltip"><span class="tooltipHeader">GAP</span>Glyceraldehyde-3-phosphate</span> and pyruvate - produced by the degradation of glucose (glycolysis) - into IPP and DMAPP. To increase the amount of IPP and DMAPP available for rubber production, we optimized the MEP pathway by elevating the levels of rate limiting enzymes in the pathway. | <span class="intro">IPP and DMAPP</span> are molecules naturally present in <span class="specialWord">E. coli</span>, produced by the bacteria through the <span class="tooltipLink">MEP pathway.</span> <span class="tooltip"><span class="tooltipHeader">MEP pathway</span>Methylerythritol phosphate pathway</span> The MEP pathway converts molecules of <span class="tooltipLink">GAP</span> <span class="tooltip"><span class="tooltipHeader">GAP</span>Glyceraldehyde-3-phosphate</span> and pyruvate - produced by the degradation of glucose (glycolysis) - into IPP and DMAPP. To increase the amount of IPP and DMAPP available for rubber production, we optimized the MEP pathway by elevating the levels of rate limiting enzymes in the pathway. | ||
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<span class="intro">Through gene manipulation</span>, we introduced the prenyltransferase gene from the rubber tree to the genetic code of <span class="specialWord">E. coli</span>. This enables the bacteria to express the prenyltransferase enzyme. In so far that the system is regulable, the bacteria should be able to convert sugar into natural rubber through; the glycolysis, the MEP pathway, and the prenyltransferase activity. Our experiments point towards this being possible. In fact, our data indicates that our bacteria do produce rubber, and we have proof of expression control. | <span class="intro">Through gene manipulation</span>, we introduced the prenyltransferase gene from the rubber tree to the genetic code of <span class="specialWord">E. coli</span>. This enables the bacteria to express the prenyltransferase enzyme. In so far that the system is regulable, the bacteria should be able to convert sugar into natural rubber through; the glycolysis, the MEP pathway, and the prenyltransferase activity. Our experiments point towards this being possible. In fact, our data indicates that our bacteria do produce rubber, and we have proof of expression control. | ||
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<span class="intro">Dig deeper</span> to see the specification and models of our system, how we designed it in detail, and what thoughts we have about safety. <br>Or go to <span class="intro">next chapter</span> to learn more about the process. | <span class="intro">Dig deeper</span> to see the specification and models of our system, how we designed it in detail, and what thoughts we have about safety. <br>Or go to <span class="intro">next chapter</span> to learn more about the process. | ||
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</html> | </html> | ||
{{:Team:SDU-Denmark/core/footer}} | {{:Team:SDU-Denmark/core/footer}} | ||
Revision as of 14:22, 26 October 2013
The production system
The micro-road to rubber
Natural rubber is a chain of several isoprene molecules. The rubber tree has a prenyltransferase PrenyltransferaseHRT2 enzyme, which links molecules of IPP IPPIsopentenyl pyrophosphate into the isoprene chain Isoprene chainnatural rubber. To start the extension of the chain, the prenyltransferase needs a DMAPP DMAPPDimethylallyl pyrophosphate molecule. Thus, in order to produce rubber, the bacteria needs the prenyltranferase, IPP, and DMAPP.
The basic idea is to convert sugar (glucose) into natural rubber through the glycolysis to yield pyruvate and GAP. These are further converted into IPP and DMAPP through the MEP pathway. A DMAPP and several IPP molecules can then be linked together into natural rubber through the action of the prenyltransferase (HRT2) from the rubber tree.
IPP and DMAPP are molecules naturally present in E. coli, produced by the bacteria through the MEP pathway. MEP pathwayMethylerythritol phosphate pathway The MEP pathway converts molecules of GAP GAPGlyceraldehyde-3-phosphate and pyruvate - produced by the degradation of glucose (glycolysis) - into IPP and DMAPP. To increase the amount of IPP and DMAPP available for rubber production, we optimized the MEP pathway by elevating the levels of rate limiting enzymes in the pathway.
Through gene manipulation, we introduced the prenyltransferase gene from the rubber tree to the genetic code of E. coli. This enables the bacteria to express the prenyltransferase enzyme. In so far that the system is regulable, the bacteria should be able to convert sugar into natural rubber through; the glycolysis, the MEP pathway, and the prenyltransferase activity. Our experiments point towards this being possible. In fact, our data indicates that our bacteria do produce rubber, and we have proof of expression control.
Dig deeper to see the specification and models of our system, how we designed it in detail, and what thoughts we have about safety.
Or go to next chapter to learn more about the process.
