Team:DTU-Denmark/Methods/Ammonium colorimetric measurements
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+ | '''Measuring the concentration of NH<sub>4</sub> in an ''E. coli''/''P.aeroginosa'' growth experiment using Spectroquant kit | ||
+ | ''' | ||
+ | |||
+ | Material: | ||
+ | * Ammonium Test Kit (#00683) | ||
+ | |||
+ | A liquid test solution should be performed to achieve a measurement between the ranges of 2-75 mg/L NH<sub>4</sub>-N and 5-150 mg/L NH<sub>4</sub>-N. | ||
+ | Ammonium solution | ||
+ | Prepare solution with 39 mg NH<sub>4</sub>-N/L: | ||
+ | * Weigh 220 mg Ammonium Bicarbonate into 100mL volumetric flask (10x concentrated) | ||
+ | * Take 5 mL of the 10x solution in 45 mL water | ||
+ | * Expected final concentration 39 mg N/L | ||
+ | |||
+ | Performing Measurements: | ||
+ | '''Ammonium''' | ||
+ | Measuring range of the kit: 2-75 mg/L NH<sub>4</sub>-N | ||
+ | # Pipette 5.0 ml of reagent 1 (NH<sub>4</sub>-1) into a test tube. | ||
+ | # Add 0.20 mL of sample when it is among the range of measuring kit (otherwise make proper dilution) | ||
+ | # Add 1 level spoon of reagent 2 (NH4-2). | ||
+ | # Shake vigorously to dissolve. | ||
+ | # Reaction time: 15 mins | ||
+ | # Pour sample into the cuvette | ||
+ | # Select method in the spectrometer by entering the AutoSelector (little black tube) | ||
+ | # Note the result | ||
+ | # If the read is above the linear range then put the sample back to the tube test, dilute it and try again. |
Revision as of 17:55, 12 August 2013
Ammonium colorimetric measurements
Ammonium colorimetric measurements
Measuring the concentration of NH4 in an E. coli/P.aeroginosa growth experiment using Spectroquant kit
Material:
- Ammonium Test Kit (#00683)
A liquid test solution should be performed to achieve a measurement between the ranges of 2-75 mg/L NH4-N and 5-150 mg/L NH4-N. Ammonium solution Prepare solution with 39 mg NH4-N/L:
- Weigh 220 mg Ammonium Bicarbonate into 100mL volumetric flask (10x concentrated)
- Take 5 mL of the 10x solution in 45 mL water
- Expected final concentration 39 mg N/L
Performing Measurements: Ammonium Measuring range of the kit: 2-75 mg/L NH4-N
- Pipette 5.0 ml of reagent 1 (NH4-1) into a test tube.
- Add 0.20 mL of sample when it is among the range of measuring kit (otherwise make proper dilution)
- Add 1 level spoon of reagent 2 (NH4-2).
- Shake vigorously to dissolve.
- Reaction time: 15 mins
- Pour sample into the cuvette
- Select method in the spectrometer by entering the AutoSelector (little black tube)
- Note the result
- If the read is above the linear range then put the sample back to the tube test, dilute it and try again.