Team:UNITN-Trento/Notebook/Labposts/07/74
From 2013.igem.org
{ "date":"2013-07-01", "author":"viola", "title":"PCR of the double terminator BBa_B0015", "content":"Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities:
B0015 in pSB1AK3 219,4 ng/ µl | B0015 in pSB1C3 413,3 ng/ µl | |
---|---|---|
H20 | 33.3 µl | 33.3 µl |
DNA | 0.5 µl | 0.5 µl |
DNTPs | 1 µl | 1 µl |
pref. FW | 2.5 µl | 2.5 µl |
suff. RV | 2.5 µl | 2.5 µl |
BUFFER | 10 µl | 10 µl |
Phusion pol. | 0.5 µl | 0.5 µl |