Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period1/Dailylog

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Revision as of 18:10, 13 September 2013 by Xiuqili (Talk | contribs)


Small Phage September - October Notebook: September 1 - September 15 Daily Log



Small Phage
March-April
May-June
July-August
September-October

9/1/13

  • Started approximately 10mL of E coli B liquid culture overnight.


9/2/13


9/3/13

  • Started approximately 20 mL of E coli B liquid culture overnight.


9/4/13

JL, LP

  • Discussed results from recent spot test for 8.26 Mutagen Concentration Test - Eighth Protocol. Phage too spread out to effectively select for mutant phage. Decided to stop until the Phage Purification Team can get the wildtype T7 phage to band.
  • Discussed plans and designs for team wiki with Darren and Keltzie.
  • Started T7 propagation to generate enough phage for the Phage Purification Team to experiment with. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock.


9/5/13

LP

  • Started approximately 10mL of E coli B liquid culture overnight.


9/6/13

JL, LP

  • Purified phage propagation sample from 9.4 via centrifugation (5 minutes at 3000 rpm) and cholorform. Spot tests were then performed to estimate phage titer.
  • Divided up assignments for updating notebook.


9/7/13

JL

  • Started approximately 15 mL E coli B liquid culture overnight.


9/8/13

JL

  • Started approximately 25mL of E coli B liquid culture overnight


9/9/13

JL, LP

  • Discussed analysis of modeling result using ImageJ.
  • Started a fresh round for phage propagation for the Phage Purification Team. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock.
  • Divided up assignments for updating the wiki.


9/10/13

JL, LP

  • Took the T1 and T2 modeling plates our the incubation at 4:30pm.
  • Started approximately 25mL of E coli B liquid culture overnight.
  • Measured plaque sizes using ImageJ.


9/11/13

JL, LP

  • Isolated the phage (from 9.9) by centrifuging down the bacteria, transferring the supernatant to a new 15mL centrifuge tube, and adding 900ul of chloroform to the supernatant.


9/12/13

  • Spot test showed plaques up to -7. This indicate that the phage suspension solution we gave the Phage Purification Team for CsCl was at least 10E9 pfu/mL.
  • Started approximately 30mL of E coli B liquid culture overnight.


8/31/13

- Further dilution series and spot test was performed to gain a more accurate idea of phage distribution in CsCl gradient. Specific procedures can be found at 8.26 Mutagen Concentration Test - Eighth Protocol.

- Started approximately 10mL of E coli B liquid culture overnight.