Team:Bielefeld-Germany/Labjournal/June

From 2013.igem.org

Revision as of 22:30, 19 September 2013 by Sgrenz (Talk | contribs)




June

Milesstones


1.Week

MFC

Mediators

Cytochromes

Biosafety

Porines









2.Week

MFC

Mediators

Cytochromes

Biosafety

Porines









3.Week

MFC

Mediators

Cytochromes

Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C

Isolation of genomic DNA from S. oneidensis

4-2006-451: Conc.:453.1 ng/µl    OD260/280: 1.92    OD260/230:2.78
4-2006-452: Conc.:447.1 ng/µl    OD260/280: 1.92    OD260/230:2.67
Dilution to PCR template: 4-2006-453: 5.5ng/µl

Amplification of the mtrCAB cluster

Protocol: Phusion 50µl Gradient
Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
Clear Bands at the expected 5.2kb

PCR-CleanUp

PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
Lane2: 4-2106-451: 7.4 ng/µl
Lane5: 4-2106-451: 8.5 ng/µl

Biosafety

Porines









4.Week

MFC

Mediators

Cytochromes

Biosafety

Porines









Retrieved from "http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/June"