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Team:Glendale CC AZ/Project
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Overall project
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)
The bacteria Deinococcus radiodurans caught our attention because its resistance to ionizing radiation, UV radiation, oxidative stress, and desiccation. Its resistance is due to its capability to repair its DNA after being exposed to these extreme conditions. Other IGEM teams have done experiments to validate the claims about Deinococcus radiodurands DNA repair capabilities. How did they do it?
The teams took several genes from Deinococcus that are involved in the DNA repair process and inserted them into E. coli. Then, they exposed the transformed E. coli to ionizing radiation, UV radiation, oxidative stress, and salt stress. The results showed that E. coli became more resistance to these stresses.
The first part of our project consists in replicating and validating two of their experiments. We will take some of the Deinococcus radiodurans genes that previous teams used, in addition to a new gene that was not used and insert them into E. coli. Then, we will expose the transformed E. coli to oxidative stress(hydrogen peroxide) and salt stress (NaCl).
The second part of our project will be our own and original contribution to IGEM. We will use an Arizona endemic species of Deinococcus, Deinococcus hopiensis. This species has not been study as much as Deinococcus Radiodurans because it is a newly discovered bacterium. Since Hopiensis and Radiodurans are two related species (belong to the same family), we want to know if hopiensis will give E. coli the same DNA repair capabilities that Radiodurans does. So, we are going to find and extract genes from Hopiensis that are homolog or have similar functions to those we previously extracted from Radiodurans. We will insert them into E. coli and exposed the transformed E. coli to oxidative and salt stress.
